Abstract
Cryopreservation plays an essential role in biobanking and cell therapy,but the physiological toxicity of traditional cryopro-tectants such as glycerol and dimethyl sulfoxide(DMSO)has raised safety issues for biomedical applications.Trehalose,a nonreducing disaccharide that accumulates in desiccation-or cold-tolerant organisms,has been considered as a biocompatible cryoprotectant.Herein,a naturally occurring membrane-active cationic peptide,melittin,was utilized to facilitate membrane-impermeable trehalose entry into cells for effective cell cryopreservation.Poly(ethylene glycol)and poly(vinyl pyrrolidone)were applied as macromolecular protectants to improve the stabilization of cell membranes.Upon the optimal protocol,the post-thaw recovery of human red blood cells in freezing bags at a hematocrit of～50％could achieve 82.9％with favorable cell morphologies and physiological functions.Furthermore,the cryosurvival of L929 fibroblasts reached 84.3％compared to the conventional method using 10％(v/v)DMSO.In short,this work by using trehalose and melittin provides a biocompatible solvent-free approach for long-term cryostorage of cells.
维普
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