兼具可打印性及生物相容性的水凝胶用于无残留打印高通量的患者来源类器官生物芯片

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中文摘要：类器官生物芯片可以模拟真实人体器官的微环境和功能特征,为疾病建模和药物筛选提供了新的平台.但传统手工培养类器官及构建生物芯片的方法通常过程复杂、成本高且耗时(至少一个月),极大地阻碍了其实际应用.本研究介绍了一种基于微针的气动打印策略,可以实现高效无残留的患者来源类器官生物芯片构建.通过开发可打印的仿生水凝胶,肿瘤活检样本可以被高效地处理成生物墨水,培养过程中细胞存活率高达92％.使用微针进行打印,生物墨水利用率超过90％,可以高效地构建类器官生物芯片并用于药物测试,在一周内给出个性化的药物筛选信息.通过微打印策略和仿生水凝胶,细胞的利用率和类器官芯片的构建效率可以有效提高,为精准医学提供了新的途径.

外文标题：Printable and biocompatible hydrogels for residual-free and high-throughput printing patient-derived organoid biochips

外文摘要：Organoid biochips can replicate the micro-en-vironment and functional traits of human organs in vitro,reflecting the physiological and pathological features of the human body.It provides a new platform for disease modeling and drug screening.However,the manual process of organoid cultivation and biochip construction using decellularized ex-tracellular matrix-based gel is typically complex,expensive,and time-consuming(at least one month),which significantly hinders practical application.Here,we introduce a micro-needle-based pneumatic printing strategy for residual-free and high-throughput construction of patient-derived orga-noid biochips.By developing printable and biomimetic hy-drogels,biopsy samples of cancer tissues can be effectively processed into discrete cells.Patient-derived colorectal cancer(CRC)cells in carboxymethylcellulose(CMC)and sodium al-ginate modified by adhesion sites exhibit high viability at 92％.Through a microneedle,the cell-ink utilization exceeds 90％.Especially,the organoid biochips can effectively be fabricated,and single cells in biochips can proliferate and differentiate into organoids with typical morphology.Finally,the patient-derived CRC organoids are used as the biochips for drug testing,which give the personalized drug screening informa-tion in a week.Overall,through the microprinting strategy and biomimetic hydrogels,the utilization rate of cells and the construction efficiency of organoid chips can be improved.This work provides a new approach for high-throughput printing patient-derived organoid biochips in precision med-icine.

外文关键词：

bioprintingbiocompatible hydrogelmicroneedleorganoid biochipdrug test

作者：

谢岱希、陈炳达、薛勇敢、孙志远、宁伯彬、张泽英、迟基梅、苏萌、宋延林

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作者单位：

Key Laboratory of Green Printing,Institute of Chemistry,Chinese Academy of Sciences,Beijing Engineering Research Center of Nanomaterials for Green Printing Technology,Beijing National Laboratory for Molecular Sciences(BNLMS),Beijing 100190,China

University of Chinese Academy of Sciences,Beijing 100049,China

Department of General Surgery,The First Medical Centre,Chinese PLA General Hospital,Beijing 100853,China

School of Chemical Engineering and Technology,Xi'an Jiaotong University,Xi'an,710049,China

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关键词：

bioprinting biocompatible hydrogel microneedle organoid biochip drug test

基金：

National Key R&D Program of ChinaNational Key R&D Program of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaChina Postdoctoral Science FoundationBeijing National Laboratory for Molecular SciencesJunior Fellow Program of Beijing National Laboratory for Molecular Sciences

项目编号：

2023YFE01115002023YFC3040900522223132207529652321006T2394480T2394484522032472022TQ0340BNLMS-CXXM-2020052021BMS20063

出版年：

2024

DOI：

10.1007/s40843-024-2933-8

中国科学:材料科学(英文)

CSTPCD

ISSN：

年,卷(期)：2024.67(8)