多功能内源性小分子衍生聚合物复合纳米粒子治疗急性败血症
Multifunctional endogenous small molecule-derived polymer composite nanoparticles for the treatment of acute sepsis therapy
高佳辉 1李慧欣 2岳芃含 1谢大洋 1李昊 3郝凯 4李艳辉 5田华雨2
作者信息
- 1. School of Materials Science and Engineering,Xiamen University of Technology,Xiamen 361024,China;State Key Laboratory of Physical Chemistry of Solid Surfaces,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China
- 2. State Key Laboratory of Physical Chemistry of Solid Surfaces,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China;Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(IKKEM),Xiamen 361005,China
- 3. School of Materials Science and Engineering,Xiamen University of Technology,Xiamen 361024,China;School of Materials Science and Engineering,Engineering Research Center of Optoelectronic Functional Materials,Ministry of Education,Changchun University of Science and Technology,Changchun 130022,China
- 4. State Key Laboratory of Physical Chemistry of Solid Surfaces,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China
- 5. School of Materials Science and Engineering,Xiamen University of Technology,Xiamen 361024,China
- 折叠
摘要
脓毒症的病情复杂,单一治疗方式效果有限,因此需要开发多功能且能快速作用的治疗药物.我们开发了一种可降解为内源性小分子、易于合成且无抗生素的多功能纳米颗粒(TL纳米颗粒)用于脓毒症的治疗.该纳米颗粒由聚硫辛酸和聚赖氨酸之间的静电相互作用形成,具有抗菌活性、吸附游离核酸和清除活性氧、活性氮物种等各种功能,为对抗脓毒症提供了更加全面的措施.本文使用CLP模型确认了TL纳米颗粒的体内治疗效果,结果表明治疗后多器官的ROS水平降低,M1促炎巨噬细胞水平降低,M2抗炎巨噬细胞水平升高.这些发现证明了TL纳米颗粒在脓毒症治疗中的有效性,其可降解特性、简单的制备过程以及迅速的治疗响应使其在临床应用中具有可观的前景.
Abstract
The complex and rapidly progressing nature of sepsis calls for the development of multifunctional and rapid-acting therapeutic agents,instead of single-modal treatments.To address this challenge,a biodegradable,easily synthesized,and antibiotic-free multifunctional nanoparticle has been created for sepsis therapy.The nanoparticle was formed by the electrostatic interaction between two endogenous small mo-lecule-derived polymers,poly(lipoic acid)and poly-lysine,and possessed various functions such as antibacterial activity,ad-sorption of cell-free DNA,scavenging of reactive oxygen and nitrogen species,providing a comprehensive approach to combating sepsis.Treatment using the cecal ligation and puncture(CLP)model confirmed the therapeutic benefits of the nanoparticles,demonstrating reduced levels of reactive oxygen across multiple organs,diminished levels of M1 pro-inflammatory macrophages,and elevated levels of M2 anti-inflammatory macrophages post-treatment.These findings emphasized the effectiveness of the nanoparticles in sepsis therapy,and properties of degradation,easy preparation,and swift therapeutic response made them promising for the fu-ture clinical applications.
关键词
sepsis therapy/antibacterial/antioxidant/cfDNA adsorptionKey words
sepsis therapy/antibacterial/antioxidant/cfDNA adsorption引用本文复制引用
出版年
2024
NETL
NSTL
万方数据