Effects of sodium pyruvate on the morphology,structure and func-tion of erythrocytes stored in vitro in type 2 diabetes rats
AIM:To observe the effect of RBC preservation solution with sodium pyruvate on the morphology,structure and function of RBC stored in vitro in type 2 diabetes rats.METHODS:Thirty SPF male SD rats,were randomly divided into 3 groups(n=10):non-T2DM conventional RBC preser-vation solution(group A),T2DM conventional RBC preservation solution(group B)and T2DM sodium pyruvate RBC preservation solution(group C).The leukoreduced RBC from the tail vein and stored for 0 d(T0),7 d(T1),14 d(T2),21 d(T3)and 28 d(T4)to detect the morphology,structure and the con-tents of 2,3-DPG,reactive oxygen species(ROS),malondialdehyde(MDA)and lactic acid(LA)of RBC in group A,B and C.The RBC stored for 14 days in vitro were labeled with PKH26,and its survival rate were tested in vivo at 1,4,10 and 16 hours after in-travenous infusion.RESULTS:At T0,the RBC mor-phology of group A was intact,which was better than that of group B and group C.With the exten-sion of storage time,the morphology of RBC in each group gradually transformed into a spindle-spherical shape.Compared with group A,the inci-dence of acanthocytes in group B and group C was higher,and the incidence of acanthocytes in group C was lower than that in group B.Compared with group A,the content of 2,3-DPG in group B and group C decreased,while ROS and MDA increased at different time points(P＜0.05).The content of 2,3-DPG in group C was higher than that in group B(P＜0.05),and the contents of ROS and MDA were lower than those in group B(P＜0.05).LA content in group B was higher than that in group A and group C(P＜0.05).At T2-T4,the LA content in group C was lower than that in group A(P＜0.05).The survival rate of RBC in group A was higher than that in group B and C,and the survival rate of RBC in group B was lower than that in group C(P＜0.05).CONCLUSION:Sodium pyruvate added RBC preser-vation solution has a certain protective effect on RBC stored in vitro in type 2 diabetic rats,and its mechanism may be related to its antioxidant effect.
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维普
