UPLC-QE-MS combined network pharmacological analysis of the mechanism of ulcerative colitis
AIM:To explore the anti-ulcerative colitis mechanism of Buzhongyiqi pills based on the network pharmacology and experimental verifica-tion.METHODS:UPLC-QE-MS was used for qualita-tive analysis of Buzhongyiqi pills.Targets of the chemistry constituents and the disease were re-trieved from GeneCards.Then the protein-protein interaction(PPI)network was constructed and core targets were screened for GO term enrichment and KEGG pathway enrichment.Ulcerative colitis mouse model was established to verify the key tar-gets.RESULTS:A total of 41 constituents migrating of Buzhongyiqi pills were identified.A total of 123 common targets of the constituents and the dis-ease and 24 core targets were screened out.KEGG enrichment and PPI network analysis showed that Buzhongyiqi pills may play a role in the treat-ment of ulcerative colitis through Akt,PI3K and oth-er pathways.Furthermore,the results of animal ex-periments showed that Buzhongyiqi pills could sig-nificantly improve the depression behaviors of ul-cerative colitis,reduce the levels of IL-6 and TNF-αin serum,inhibition Akt/PI3K signaling,and reduce the protein expression of PI3K.CONCLUSION:Bu-zhongyiqi pills may play a role in the treatment of ulcerative colitis by inhibition Akt/PI3K signaling pathway,and inhibiting PI3K and reduce the levels of IL-6 and TNF-α in the mice.
国家科技期刊平台
NSTL
万方数据
