Transcriptomic analysis of the molecular mechanism of Tiaopi Chengqi decoction improving gastric digestive function in mice with food accumulation
AIM:To explore the molecular mecha-nism of Tiaopi Chengqi decoction(TpCqD)improv-ing hyperthermia and high-protein food-induced hy-perphagia mice based on transcriptomics.METH-ODS:C57 mice were randomly divided into a con-trol group,model group,low-dose TpCqD group,high-dose TpCqD group,and domperidone group.The general condition of the experimental mice was observed and the average food intake was counted,and the rate of gastric emptying and intes-tinal propulsion was determined for each group of mice.H&E staining was used to observe pathologi-cal changes in gastric tissue.PAS staining was used to observe glycogen changes in gastric tissue.Pep-sin activity was determined by colorimetry.pH val-ue of gastric contents was measured by acid-base titration.Transcriptome sequencing was used to an-alyze the differential genes in gastric tissue,a volca-no map and a cluster heat map were made for the differential genes,and KEGG was used to analyze the signal pathway enrichment of the differential genes.RT-qPCR verified the differential genes ob-tained by screening.RESULTS:After treatment with TpCqD,the body weight and average food intake of mice with food accumulation increased(P＜0.05),and the intestinal propulsion rate and gastric emp-tying speed of mice with food accumulation accel-erated(P＜0.05).TpCqD could protect gastric tissue structure and glycogen degradation,increase pep-sin activity(P＜0.05),and reduce gastric content pH(P＜0.05).Transcriptome results showed that TpCqD could regulate the expression of Acox2 and cilp2,regulate fat digestion and absorption,protein diges-tion and absorption,and pancreatic secretion sig-nals.RT-qPCR showed that compare with model group,TpCqD up-regulated Acox2(P＜0.05)and down-regulated the mRNA level of cilp2(P＜0.05).CONCLUSION:TpCqD ameliorated digestive dys-function in mice with high-calorie and high-protein diets leading to food accumulation involving the regulation of the fat and sugar metabolism genes Acox2 and cilp2,and pancreatic secretory signaling.
Tiaopi Chengqi decoctiontranscrip-tomedyspepsiagastrointestinal function
国家科技期刊平台
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