首页|皖南蝮蛇毒抑瘤组分-Ⅰ对胃癌MKN-28细胞增殖、迁移及凋亡的影响

皖南蝮蛇毒抑瘤组分-Ⅰ对胃癌MKN-28细胞增殖、迁移及凋亡的影响

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  • 国家科技期刊平台
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目的:探讨皖南蝮蛇毒抑瘤组分-Ⅰ(agkis-trodon halys venom antitumor component Ⅰ，AH-VAC-Ⅰ)对胃癌MKN-28细胞生物学行为的影响作用。方法:不同实验浓度(5、10和15 μg/mL)AHAVC-Ⅰ处理胃癌细胞MKN-28 24 h后，采用细胞增殖和毒性检测(cell counting kit-8，CCK-8)法检测细胞增殖活力;划痕实验和Tanswell小室检测细胞迁移能力;激光共聚焦显微镜(annexin V-mCherry/DAPI双染)和流式细胞术(annexin V-FITC/PI双染)检测细胞凋亡;Western blot检测细胞凋亡相关蛋白Caspease-3的表达水平。结果:AHVAC-Ⅰ各浓度组分别与正常对照组相比结果显示，随着AHVAC-Ⅰ浓度的增加，MKN-28细胞增殖活力逐渐下降(P＜0。01)，细胞迁移能力逐渐被抑制(P＜0。01)，细胞凋亡率增高(P＜0。05)，凋亡相关蛋白 Caspease-3表达上调(P＜0。01)。结论:AHVAC-Ⅰ抑制胃癌细胞MKN-28增殖和迁移，诱导其凋亡。
Effects of the proliferation,migration and apoptosis of AHVAC-Ⅰ on gastric cancer MKN-28 cells
AIM:To investigate the effects of ag-kis-trodon halys venom anti-tumor component Ⅰ(AHVAC-Ⅰ)on the biological behavior of gastric can-cer MKN-28 cells.METHODS:Gastric cancer MKN-28 cells were treated with the experimental con-centrations(5,10,15 μg/mL)of AHAVC-Ⅰ for 24 h.Cell proliferation and toxicity assay(cell counting kit-8,CCK-8)was used to detect the inhibition rates of the cells in different concentrations of AHVAC-Ⅰ.The migration ability of the cells was evaluated by wound-healing and Transwell assay.The apoptosis were observed by laser confocal microscopy with annexin V-mCherry/DAPI double staining,and the apoptosis rates were analyzed by flow cytometry with annexin V-FITC/PI double fluorescence stain-ing.The protein level of Caspease-3 was deter-mined by Western blot.RESULTS:Compared with normal control group,the results of AHVAC-Ⅰ con-centration groups showed that with the increase of AHVAC-Ⅰ concentration,the proliferative activity of MN-28 cells decreased gradually(P＜0.01),the cell migration ability decreased gradually(P＜0.01),and the cell apoptosis rate increased(P＜0.05).The ex-pression of apoptosis-related protein Caspease-3 was up-regulated(P＜0.01).CONCLUSION:AHVAC-Ⅰinhibits proliferation and migration of gastric can-cer MSN-28 cells and induces apoptosis.

agkis-trodon halys venom antitumor component-Ⅰ(AHVAC-Ⅰ)gastric cancercell prolifer-ationcell migrationcell apoptosis

黄小梅、支慧、陈浩、卢林明、朱晓群、王丽珍、周珏、庞金金、许金亮

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皖南医学院病理解剖学教研室,芜湖 241002,安徽

皖南医学院临床医学院,芜湖 241002,安徽

AHVAC-Ⅰ 胃癌 细胞增殖 细胞迁移 细胞凋亡

安徽省教育厅自然科学研究重点项目皖南医学院大学生科研资助金项目活性生物大分子研究安徽省重点实验室自主研究课题安徽省高等学校质量工程项目

KJ2020A0611WK2020S01LAB202004S202010368107

2024

10.12092/j.issn.1009-2501.2024.03.004

中国临床药理学与治疗学
中国药理学会

中国临床药理学与治疗学

CSTPCD北大核心
影响因子：0.97
ISSN：1009-2501
年,卷(期)：2024.29(3)
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