皖南蝮蛇毒抑瘤组分-Ⅰ对胃癌MKN-28细胞增殖、迁移及凋亡的影响
Effects of the proliferation,migration and apoptosis of AHVAC-Ⅰ on gastric cancer MKN-28 cells
黄小梅 1支慧 1陈浩 1卢林明 1朱晓群 1王丽珍 1周珏 1庞金金 2许金亮2
作者信息
- 1. 皖南医学院病理解剖学教研室,芜湖 241002,安徽
- 2. 皖南医学院临床医学院,芜湖 241002,安徽
- 折叠
摘要
目的:探讨皖南蝮蛇毒抑瘤组分-Ⅰ(agkis-trodon halys venom antitumor component Ⅰ,AH-VAC-Ⅰ)对胃癌MKN-28细胞生物学行为的影响作用.方法:不同实验浓度(5、10和15 μg/mL)AHAVC-Ⅰ处理胃癌细胞MKN-28 24 h后,采用细胞增殖和毒性检测(cell counting kit-8,CCK-8)法检测细胞增殖活力;划痕实验和Tanswell小室检测细胞迁移能力;激光共聚焦显微镜(annexin V-mCherry/DAPI双染)和流式细胞术(annexin V-FITC/PI双染)检测细胞凋亡;Western blot检测细胞凋亡相关蛋白Caspease-3的表达水平.结果:AHVAC-Ⅰ各浓度组分别与正常对照组相比结果显示,随着AHVAC-Ⅰ浓度的增加,MKN-28细胞增殖活力逐渐下降(P＜0.01),细胞迁移能力逐渐被抑制(P＜0.01),细胞凋亡率增高(P＜0.05),凋亡相关蛋白 Caspease-3表达上调(P＜0.01).结论:AHVAC-Ⅰ抑制胃癌细胞MKN-28增殖和迁移,诱导其凋亡.
Abstract
AIM:To investigate the effects of ag-kis-trodon halys venom anti-tumor component Ⅰ(AHVAC-Ⅰ)on the biological behavior of gastric can-cer MKN-28 cells.METHODS:Gastric cancer MKN-28 cells were treated with the experimental con-centrations(5,10,15 μg/mL)of AHAVC-Ⅰ for 24 h.Cell proliferation and toxicity assay(cell counting kit-8,CCK-8)was used to detect the inhibition rates of the cells in different concentrations of AHVAC-Ⅰ.The migration ability of the cells was evaluated by wound-healing and Transwell assay.The apoptosis were observed by laser confocal microscopy with annexin V-mCherry/DAPI double staining,and the apoptosis rates were analyzed by flow cytometry with annexin V-FITC/PI double fluorescence stain-ing.The protein level of Caspease-3 was deter-mined by Western blot.RESULTS:Compared with normal control group,the results of AHVAC-Ⅰ con-centration groups showed that with the increase of AHVAC-Ⅰ concentration,the proliferative activity of MN-28 cells decreased gradually(P＜0.01),the cell migration ability decreased gradually(P＜0.01),and the cell apoptosis rate increased(P＜0.05).The ex-pression of apoptosis-related protein Caspease-3 was up-regulated(P＜0.01).CONCLUSION:AHVAC-Ⅰinhibits proliferation and migration of gastric can-cer MSN-28 cells and induces apoptosis.
关键词
AHVAC-Ⅰ/胃癌/细胞增殖/细胞迁移/细胞凋亡Key words
agkis-trodon halys venom antitumor component-Ⅰ(AHVAC-Ⅰ)/gastric cancer/cell prolifer-ation/cell migration/cell apoptosis引用本文复制引用
基金项目
安徽省教育厅自然科学研究重点项目(KJ2020A0611)
皖南医学院大学生科研资助金项目(WK2020S01)
活性生物大分子研究安徽省重点实验室自主研究课题(LAB202004)
安徽省高等学校质量工程项目(S202010368107)
出版年
2024
国家科技期刊平台
NSTL
万方数据