Effects of osthole on renal oxidative stress injury in aged spontane-ously hypertensive rats by regulating PI3K/Akt/mTOR signaling path-way
AIM:To explore the mechanism of ost-hole on elderly spontaneously hypertensive rats.METHODS:20-month-old spontaneously hyperten-sive rats(SHRs)and healthy Wistar-Kyoto(WKY)rats were purchased.SHRs were treated with ost-hole(i.g.)for 8 weeks.The systolic blood pressure and diastolic blood pressure of rats were moni-tored.Hematoxylin-eosin staining(H&E),periodic acid-schiff staining(PAS)and Masson staining were used to observe the pathological changes of rat kid-ney tissues.The activity of superoxide dismutase(SOD),malondialdehyde(MDA)and glutathione(GSH)in rat kidney was detected by ELISA kit.PI3K/Akt/mTOR signaling pathway related proteins were detected by western blot.RESULTS:Osthole re-duced the systolic and diastolic blood pressure of SHRs,improved the histopathological changes of SHRs kidney,reduced the activity of MDA in SHRs kidney,and increased the activity of SOD and GSH.Osthole reduced the levels of p-PI3K,p-Akt and p-mTOR.CONCLUSION:Osthole reduces the activity of PI3K/Akt/mTOR signaling pathway and exerts a protective effect on renal oxidative stress injury in aged spontaneously hypertensive rats.
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