AIM:To explore the mechanism of Periplaneta americana extracts(PA-CⅡ-3)on anti-prostate cancer.METHODS:Prostate cancer cells PC3 in logarithmic growth phase were divided into a control group and an experimental group.The PA-CⅡ-3 solutions of 1 000,1 200,1 400,1 600,1 800,and 2 000 μg/mL were added successively to the PC3 cells in experimental group,for screening out the appropriate drug concentration and best time to intervene by CCK-8 assay.And then,PA-CⅡ-3 so-lution at 1 400 μg/mL was continuously intervened in PC3 cells for 72 hours.The cell proliferation abili-ty was detected by CCK-8 assay and plate colony formation assay,the cell migration ability was test-ed by scratch wound assay,and the mRNA and pro-tein expressions of ZAG were detected by Real time qPCR and Western blot methods,respectively.RE-SULTS:After PA-CⅡ-3 intervention,the proliferation and migration abilities of PC3 cells were significant-ly inhibited,and the mRNA and protein expression levels of ZAG in PC3 cells were promoted.CONCLU-SION:PA-CⅡ-3 can play the role of anti-prostate cancer by inhibiting the proliferation and migration of PC3 cells,and upregulating ZAG.
关键词
美洲大蠊提取物/前列腺癌/锌α2糖蛋白/细胞增殖/细胞迁移
Key words
Periplaneta americana extracts/pros-tate cancer/zinc-alpha2-glycoprotein/cell prolifera-tion/cell migration
维普
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