大鼠肝纤维化、肝硬化、小肝癌MRI扩散参数与Ki-67、PCNA表达的相关性
Correlation between MRI diffusion parameters and Ki-67 and PCNA expression in rat liver fibrosis,cirrhosis and small liver cancer models
王国光 1孟令平 1胡明秀 1官兵 1梁爽 1洪玉萍1
作者信息
- 1. 上海市第六人民医院金山分院,上海 201599
- 折叠
摘要
目的:探讨MRI扩散加权成像(Diffusion weighted imaging,DWI)在大鼠肝纤维化、肝硬化、小肝癌动物模型中的诊断价值,并分析ADC值和eADC值与细胞增殖标志物Ki-67、PCNA表达的相关性.方法:100 只清洁级SD大鼠,随机分为 2组,实验组 85 只,对照组 15 只,实验组腹腔定点注射硫代乙酰胺(TAA),对照组替代采用生理盐水,300 mg/kg,3 次/周.MRI-DWI采用单次激发自旋回波-回波平面成像(SE-EPI)序列,取b=300 s/mm2、600 s/mm2 两组,生成ADC图及eADC 图并测量ADC及eADC值;按组织病理学进行肝纤维化、肝硬化、小肝癌分级,并与ADC值、eADC值和细胞增殖标志物Ki-67、PCNA表达进行Pearson相关分析.结果:共有 55 只大鼠(实验组 41 只,对照组 14 只)成功获取DWI参数数据,成功率 55%(55/100).随着肝纤维化逐步进展,DWI显示实验组大鼠肝内信号逐渐增高,高b值图像较明显,但图像质量较差.ADC值与大鼠肝纤维化、肝硬化、小肝癌病理分级之间呈负相关(P<0.05),而eADC值则为正相关(P<0.05);Ki-67、PCNA在肝纤维化、肝硬化期表达不明显,在小肝癌期相对较为明显,ADC值与Ki-67、PCNA表达呈负相关(P<0.05),eADC值与Ki-67、PCNA 表达呈正相关(P<0.05).结论:MRI-DWI参数ADC、eADC值能够反映肝纤维化、肝硬化、小肝癌逐步发展的规律,与细胞增殖标志物Ki-67、PC-NA表达之间有一定相关性,能够为临床预测小肝癌的发生提供一定的参考依据.
Abstract
Objective:To explore the diagnostic value of MRI diffusion weighted imaging(DWI)in rat liver fibrosis,cirrho-sis,and small liver cancer animal models,and to analyze the correlation between ADC and eADC values with cell prolifera-tion markers Ki-67 and PCNA expression.Methods:One hundred clean grade SD rats were randomly divided into two groups:the experimental group(n=85)and the control group(n=15).The experimental group received intraperitoneal injection of thioacetamide(TAA)at a fixed point,while the control group was replaced with physiological saline,300 mg/kg,3 times per week.MRI-DWI used the single shot spin echo echo planar imaging(SE-EPI)sequence,taking groups b=300s/mm2 and 600s/mm2,to generate ADC and eADC map and measure ADC and eADC values;Liver fibrosis,cirrhosis,and small liver cancer were classified according to histopathology,and correlation analysis was performed with ADC value,eADC value,and cell prolifera-tion markers Ki-67 and PCNA expression.Results:DWI parameter data of 55 rats(experimental group(n=41),control group(n= 14))was successfully obtained DWI parameter data,with a success rate of 55%(55/100).As liver fibrosis gradually progresses,DWI showed that the signal in the liver of the experimental group mice gradually increased,and high b-value images were more obvious,but the image quality was poor.The ADC value was negatively correlated with the pathological grading of liver fibrosis,cirrhosis,and small liver cancer in rats(P<0.05),while the eADC value was positively correlated(P<0.05);Ki-67 and PCNA did not significantly express in liver fibrosis and cirrhosis,but relatively more pronounced in small liver cancer.The ADC value is negatively correlated with Ki-67 and PCNA expression(P<0.05),while the eADC value is positively correlated with Ki-67 and PCNA expression(P<0.05).Conclusion:The ADC and eADC values of MRI-DWI can reflect the progressive development of liver fibrosis,cirrhosis,and small liver cancer.There is also a certain correlation between MRI-DWI parame-ters and cell proliferation markers Ki-67 and PCNA expression,which can provide a certain reference basis for clinical pre-diction of the occurrence of small liver cancer.
关键词
肝纤维化/肝硬化/肝肿瘤/磁共振成像Key words
Hepatic Fibrosis/Liver Cirrhosis/Liver Neoplasms/Magnetic Resonance Imaging引用本文复制引用
基金项目
上海市卫生健康委员会科研课题项目(201940176)
出版年
2024
国家科技期刊平台
NSTL
万方数据