Objective:To explore the value of the Layer-specific strain technique in the evaluation of left ventricular sys-tolic function in patients with acute viral myocarditis(AVMC).Methods:Thirty-seven patients diagnosed with AVMC and left ventricular ejection fraction(LVEF)≥50%in our hospital from January 2020 to February 2023 were prospectively selected as the case group and 37 healthy volunteers during the same period were randomly selected as the control group.The conven-tional echocardiography parameters of the two groups were compared,and the conventional echocardiography parameters and cardiac magnetic resonance(CMR)parameters of the case group were compared and analyzed.Global longitudinal strain(GLS)and global circumferential strain(GCS)of the left ventricular three-layers myocardium during systole in both groups were mea-sured by the Layer-specific strain technique,and the differences between the groups and their correlation with CMR parame-ters were analyzed.Results:There was no significant difference in routine echocardiographic parameters between the two groups(P>0.05).The routine echocardiography underestimated left ventricular end-diastolic volume(LVEDV),end-systolic volume(LVESV)and stroke volume(SV)of the case group compared with CMR(P<0.05).The absolute values of the GLS and GCS of the three-layers myocardium in the case group were obviously lower than those in the control group(P<0.001),and were nega-tively correlated with the visual presence score(VPS)of CMR parameters(all P<0.001),and positively correlated with LVEF(all P<0.01).Conclusion:The Layer-specific strain technique has certain clinical value in early identification of left ventricular systolic function damage in patients with AVMC.