Objective:To evaluate the diagnostic value of transvaginal ultrasound(TVS)three-step system ultrasound in deep infiltrating endometriosis(DIE).Methods:Fifty-seven patients with suspected DIE underwent surgery in our hospital.All patients underwent TVS three-step system ultrasound evaluation before surgery.Detailed medical history was asked before surgery,the location,size and characteristic ultrasound manifestations of the lesions were accurately recorded,and the results were compared with surgery and/or histology.Results:According to the different locations and sizes of the lesions,the sensi-tivity of TVS three-step system ultrasound for evaluating DIE ranged from 55.3%to 99.7%,and the specificity ranged from 84.7%to 96.6%.Conclusion:The TVS three-step method has value in detecting the location,range and depth of DIE,which is helpful for comprehensive assessment of DIE patients before surgery.