Therapeutic effect of Pine pollen,Scutellaria baicalensis and their compat-ibility on acute liver injury mice and the hemostatic effect of traumatic in-jury
Objective To explore the therapeutic effect and hemostatic effect of Pine pollen and scutellaria baicalensis and their combination on acute liver injury in mice.Methods The mice were randomly divided into 6 groups:the control group,Pine pol-len group,Scutellaria baicalensis group,and the Pine pollen and Scutellaria baicalensis 1:1 ratio low,medium and high dose experi-mental(low,medium and high dose)groups.The control group was given physiological saline,the Pine pollen group and the Scutellar-ia baicalensis group were given 1.5 g/kg corresponding extracts,and low,medium and high dose groups were given 1.5,4.5,7.5 g/kg corresponding extracts,rats were given by intragastric administration for 21 d.1 h after the last administration,mice models of acute liver injury of carbon tetrachloride(CCl4)and alcohol were established.After fasting without water for 12 h,blood which taken from the eyeballs was to test liver function indexes,and the free liver was weighed to calculate the liver weight coefficient.In addition,healthy mice were taken to study the traumatic hemostas effect of Pine pollen and Scutellaria baicalensis and their compatibility by measuring the time of hemostasis by tail docking,femoral artery hemostasis and liver hemostasis.Results Compared with the control group,Pine pollen group and Scutellaria baicalensis group showed good hepatoprotective and hemostatic effects(P＜0.05).Compared with Pine pollen group and Scutellaria baicalensis group,liver function and hemostatic effect were significantly improved by compatibili-ty group(P＜0.05).Conclusions Pine pollen,Scutellaria baicalensis and their compatibility have a good hepatoprotective and hemo-static effects,and their effects are further strengthened with the increase of concentration.Pine pollen and Scutellaria baicalensis show a good synergistic effect on improving liver function and hemostasis time.
Pine pollenScutellaria baicalensisCompatibilityAcute liver injuryCarbon tetrachloride(CCl4)AlcoholHemostasis
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