肺癌A549细胞来源的IL-17A对破骨细胞分化的作用及分子机制
The effect and molecular mechanism of IL-17A derived from lung cancer A549 cells on osteoclast differentiation
王洪凯 1唐浩 1袁术杰 1梁春涛1
作者信息
- 1. 桂林医学院第二附属医院骨科,广西 桂林 541199
- 折叠
摘要
目的 研究肺癌A549细胞来源的白细胞介素(IL)-17A对破骨细胞分化的作用及分子机制.方法 利用慢病毒基因干扰技术建立低表达IL-17A的A549细胞(A549-si).将A549细胞与破骨细胞前体细胞共培养,观察A549-si对破骨细胞分化的影响;荧光定量聚合酶链反应(qPCR)检测A549-si对破骨细胞分化相关基因和IL-8 mRNA表达的影响;酶联免疫吸附试验(ELISA)检测A549-si对IL-8蛋白表达的影响;将IL-8抗体作用于A549细胞与破骨细胞共培养模型,观察IL-8抗体能否减弱A549细胞对破骨细胞分化的促进作用.结果 A549细胞与破骨细胞前体细胞共培养可以明显促进破骨细胞分化,A549-si能够明显抑制A549细胞对破骨细胞分化的促进作用并明显抑制破骨细胞分化相关基因表达(P＜0.05);A549-si能够明显抑制A549细胞IL-8 mRNA表达和水平(P＜0.05);IL-8抗体能够明显逆转A549细胞对破骨细胞分化的促进作用(P＜0.05).结论 A549细胞来源的IL-17A通过促进IL-8表达促进破骨细胞分化.
Abstract
Objective To study the effect of interleukin(IL)-17A derived from lung cancer A549 cells on the differentiation of osteoclast and its molecular mechanism.Methods Lentivirus interference technology was used to construct A549 cells with low expres-sion of IL-17A(A549-si).A549 cells were co-cultured with osteoclast precursor cell to observe the effect of A549-si on osteoclast dif-ferentiation.The effect of A549-si on the expression of osteoclast differentiation related genes and IL-8 mRNA was detected by fluores-cent quantitative polymerase chain reaction(qPCR).The effect of A549-si on IL-8 protein expression was detected by enzyme linked immunosorbent assay(ELISA).IL-8 antibody was applied to the co-culture model of A549 cells and osteoclast to observe whether IL-8 antibody could weaken the promoting effect of A549 cells on osteoclast differentiation.Results The differentiation of osteoclast was sig-nificantly promoted by co-culture of A549 cells with osteoclast precursor cell.The promotion of A549 cells on osteoclast differentiation and the expression of osteoclast differentiation related genes were significantly inhibited by A549-si(P＜0.05).The expressions of IL-8 mRNA and protein in A549 cells were significantly inhibited by A549-si;the promoting effect of A549 cell on osteoclast differentiation was significantly reversed by IL-8 antibody(P＜0.05).Conclusions IL-17A derived from A549 cells could promote the differentiation of osteoclast by promoting the expression of IL-8.
关键词
白细胞介素(IL)-17A/非小细胞肺癌/IL-8/破骨细胞分化Key words
Interleukin(IL)-17 A/non-small cell lung cancer/IL-8/osteoclast differentiation引用本文复制引用
出版年
2025
NETL
NSTL
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