In this study, two different approaches of regeneration systems for explants in Eucalyptus pellita have been taken to establish an efficient differentiation and regeneration protocols. The somatic embryogenesis from in vitro hypocotyls and cotyledons in Eucalyptus pellita has been investigated. The typical three kinds of callus types were induced from hypocotyls and cotyledons cultured on the 2,4-D containing medium with BA or kinetin.High frequency (82 % ) plantlet production from the friable, granular embryogenic callus derived from hypocotyls has been obtained on the differentiation medium( 1μM TDZ; 1μM BA plus 0. ltMNAA ) developed in this study.The relationship between the callus types formed on different callus induction media and their corresponding differ entiation rate was further discussed. In addition, plant regeneration through organogenesis from in vitro hypocot yls, cotyledons and mature leaves in Eucalyptus pellita has also been conducted with the modified basal B5 medi um supplemented with a range of combinations of BA and NAA. The highest frequency of shoot formations for three kinds of explants varied on the different regeneration medium, with the regeneration rate for leaves, cotyle dons and hypocotyls in ascending order: 41.67%, 66.67% and 83.33%, respectively. The significance of the two different plant regeneration systems was discussed. To our knowledge, this is the first report on the regenera tion of Eucalyptus pellita.
国家科技期刊平台
NSTL
万方数据
维普
