Impacts of folic acid on the levels of SOD,GSH-Px,GSH and MDA in non-alcoholic fatty liver rats
Objective To investigate the impacts of folic acid on the levels of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),glutathione(GSH),and malondialdehyde(MDA) in non-alcoholic fatty liver disease (NAFLD) rats.Methods Sprague-Dawley(SD) rats were fed with high-fat diet for 8 weeks to construct a NAFLD model.They were randomly separated into the model group,low-dose folic acid group(10 mg/kg),medium-dose folic acid group(20 mg/kg),high-dose folic acid group(40 mg/kg),and Orlistat group(60 mg/kg positive control drug),with 10 rats in each group.Another 10 SD rats were fed with normal diet for 8 weeks were included as the control group. After treatment with folic acid and Orlistat,the levels of total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),triglyceride(TG),and free fatty acid(FFA) in rat blood lipids,and liver function indicators like alanine aminotransferase (ALT),γ-glutamyl transpeptidase(γ-GT),and aspartate aminotransferase(AST) were detected.H&E staining and Oil Red O staining were respectively applied to detect the morphology and steatosis of rat liver tissue.The NAFLD activity scores of each group were compared.Reagent kits were applied to detect the levels of SOD,GSH-Px,GSH,and MDA in rat serum and liver tissue.Results Compared with those of the control group,rats in the model group showed obvious steatosis and pathological damage in the liver,significantly higher serum levels of TC,LDL-C,TG,FFA,ALT,γ-GT and AST,NAFLD activity score,and MDA in serum and liver tissue,but significantly lower SOD,GSH-Px,and GSH in serum and liver tissue(P<0.05).Compared with those of the model group,rats in the low,medium,high-dose folic acid groups had reduced steatosis and pathological damage in the liver tissue,significantly lower serum levels of TC,LDL-C,TG,FFA,ALT,γ-GT and AST,NAFLD activity score,and MDA in serum and liver tissue,but significantly higher SOD,GSH-Px,and GSH in serum and liver tissue in a dose-dependent manner(P<0.05).Compared with the high-dose folic acid group,all indicators were similar to the Orlistat group(P>0.05).Conclusion Folic acid can reduce blood lipid levels,enhance antioxidant enzyme activity,lower lipid peroxidation levels,and inhibit oxidative stress in NAFLD rats,thereby reducing hepatic steatosis,pathological damage,and improving liver function in rats.
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