Given the complex structure,large differences in reservoir distribution,and unclear gas reser-voir enrichment patterns in the Ordos Basin,this article employs the fluorescence quantitative PCR meth-od of microbial gene detection technology to measure the abundance of oil and gas indicator genes in the samples of the study area.Additionally,high-throughput gene sequencing is used to detect the types of hydrocarbon microbial populations in the samples.Analysis indicates three major hydrocarbon anomaly zones in the northeastern,western,and southern parts of this area.The distribution characteristics are consistent with sedimentary understanding,and there is a close relationship between the western and southern zones.The northeast zone is unrelated to the other two,possibly due to the escape of hydrocar-bons from different layers of tight gas and coalbed methane.Actual drilling shows that this technology has good applicability in the evaluation of oil and gas in the mixed-source area of tight gas and coalbed meth-ane in the Ordos Basin,providing a reference for the screening of favorable oil and gas zones.
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