Bioinformatics and Expression Analysis of the StNF-YA9Gene in Potato
NF-Y is a transcription factor ubiquitous in eukaryotes,typically functioning as NF-YA/B/C heterotrimers.The NF-YA transcription factor regulates the expression of downstream target genes in response to stress by specifically binding to the CCAAT box in the DNA promoter region.It was discovered that the potato exhibits increased sensitivity to drought stress when the StNF-YA9 gene is silenced through a comprehensive analysis of the potato NF-Ys gene family.In this research,the StNF-YA9 gene was cloned from the potato variety'Atlantic'.Bioinformatics analysis showed that the total length of StNF-YA9 gene was 1 231 bp,and the CDS region length was 723 bp.The subcellular localization fusion expression vector of StNF-YA9 was constructed to determine its localization in the nucleus.The expression pattern of StNF-YA9 in potato tissues and under stress was analyzed by qRT-PCR.The results showed that the relative expression of the StNF-YA9 gene was highest in leaves and lowest in buds.The expression was up-regulated by polyethylene glycol(PEG)and abscisic acid(ABA).The results would provide the theoretical basis for subsequent functional studies on the potato StNF-YA9 gene.
NETL
NSTL
万方数据
