Antifungal Activity of Some Chinese Herbal Medicine Extracts Against Phytophthora Infestans
The application of Chinese herbal medicine to the prevention and control of crop diseases and pests has attracted much attention due to their unique advantages.To explore the efficient Chinese herbal medicine preparations that can be used for controlling Phytophthora infestans,and help green prevention and control of P.infestans,the ternary solvent of anhydrous ethanol,petroleum ether and ethyl acetate(volume ratio 4∶3∶3)was chosen to extract different parts of 11 Chinese herbal medicines,for instance,Thymus mongolicus,by cold impregnation method to obtain the corresponding preparations.The inhibitive activity of 11 Chinese herbal medicine preparations against P.infestans in vitro and semi in vivo was determined by plate mycelium growth rate and leaf inoculation method,respectively.The preparations of T.mongolicus and Rheum palmatum had strong antibacterial activity,with EC50 of 130.19 and 364.90 mg/kg,respectively,which were much stronger than those of eugenol(669.52 mg/kg).The preparations of Saposhnikovia divaricata,Rumex dentatus,Pogostemon cablin,Cnidium monnieri,Daucus carota and Cullen corylifolium were also strong for antibacterial activity,with EC50 of 768.28,807.33,874.75,953.52,1 519.11 and 1 659.58 mg/kg,respectively,which were slightly lower or lower than eugenol.It is evident that T.mongolicus,R.palmatum,S.divaricata,R.dentatus,P.cablin,C.monnieri,D.carota and C.corylifolium exhibit strong control effects and potential application value for P.infestans.
Chinese herbal medicineextractsPhytophthora infestansantifungal activity
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