Mechanism of the Medicago Sativa Extract Against Ultraviolet and Blue Light Induced Photodamage
Objective To explore the effect and mechanism of Medicago Sativa extract(MS)against light damage through various evaluation methods.Methods The ABTS free radical detection model was built in vitro,the ROS free radical detection model was produced by high energy visible light(HEVL)irradiation on keratinocytes(HaCaT),the cell viability,production of prostaglandin E2(PGE2)and matrix metalloproteinase-1(MMP-1)models after UV irradiation on HaCaT,and the human skin injury model induced by HEVL were used to verify the efficacy of MS.Results MS had a good antioxidant capacity.The IC50 of MS scavenging ABTS free radicals was 0.048%.MS with a mass fraction of 0.25%-0.5%could significantly reduce ROS production in HaCaT cells exposed to HEVL.MS with a mass fraction of 0.1%-0.3%increased the cell survival rate in UV irradiation injury model in a dose-dependent manner,and decreased the expression of prostaglandin E2(PGE2)and matrix metalloproteinase-1(MMP-1).In the human skin injury model induced by HEVL,MS with a mass fraction of 0.5%-5%can significantly reduce the formation of skin erythema,increase skin brightness and reduce the formation of melanin.Conclusion As a kind of anti photodamage raw material from plants,MS can be used in the products claimed to have soothing,repairing and anti wrinkle effects.
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