Study on the Effect of Shijingwan on LCs and Inflammatory Factors in Photoaging Rats
Objective To investigate the effects of Shijingwan on the levels of skin Langerhans cells(LCs)and inflammatory cytokines in photoaged rats.Methods 50 female SD rats were randomly divided into model group,positive control group,Shijingwan low-dose group,Shijingwan medium-dose group and Shijingwan high-dose group,with 10 rats in each group.The skin photoaging model was established,and Shijingwan were given for intervention.After the experiment,the amount and morphology of LCs in the skin of rats were observed by immunohistochemistry,the degree of inflammatory infiltration was observed by pathological sections,and the contents of Interleukin-6(IL-6)and Tumor necrosis factor-α(TNF-α)in the skin tissue and serum were detected by ELISA.Results Immunohistochemical results showed that,compared with model group,the number of epidermal LCs cells in each dose group of Shijingwan increased,the morphology of cell bodies improved,the formation of dendritic structure increased,and the connections between cells were tight.The improvement effect of Shijingwan high dose group was more significant.Pathological section results showed that the degree of skin inflammatory cell infiltration was improved in each dose group of Shijingwan,and the degree of improvement was more obvious in the high dose group of Shijingwan.ELISA results showed that the contents of inflammatory cytokines IL-6 and TNF-α in skin homogenate and serum of Shijingwan were decreased in each dose group.There was significant decrease in the medium and high dose groups of Shijingwan,with statistical significance(P＜0.01),but no significant difference compared with positive control group(P＞0.05).Conclusion Shijingwan can increase the number of LCs cells in the skin tissue of photoaging rats,improve the abnormal morphology of LCs cells,reduce the degree of inflammatory cell infiltration in the skin tissue of rats,and reduce the contents of inflammatory factors IL-6 and TNF-α in the skin tissue and serum of rats,indicating that Shijingwan can improve the skin immune function,inhibit inflammatory factors,and repair the skin function.Improve skin aging.
万方数据
维普
