Effect of Photodynamic Therapy on Migration and Invasion of Two Melanoma Cells and Mechanism of Action
Objective A preliminary investigation of the effects and mechanisms of photodynamic therapy(PDT)on the migration and invasion of two types of melanoma cells.Methods Melanoma cells A375 and B16-F10,which grew to the log stage,were digested and centrifuged by pancreatic enzyme,counted,and inoculated into six-well plates.After culture,cell fusion reached more than 80%to 90%,the cells were incubated with photosensitizer 1 mmol/L 5-aminoketovalerate(5-ALA)for 2 h,and then treated with 635 nm red light irradiation.According to the different irradiation energy,it was divided into 0 J(control group),2.4J(PDT 2.4J group),4.8J(PDT 4.8J group).Scratch healing test and transwell invasion test were used to detect the effects of photodynamic therapy on the migration and invasion of melanoma cell lines A375 and B16-F10.Western blotting was used to detect the relative expression of migration and invasion-related proteins[Ki-67,vascular endothelial growth factor(VEGF),matrix metalloproteinase 9(MMP9)].Results The results of cell scratches and Transwell experiments showed that after 24 hours of PDT treatment,compared with the control group,the scratch healing rate and the invasion of cells in the PDT 2.4 J group and PDT 4.8 J group were in the B16-F10 and A375 cell lines.The number was significantly reduced(P<0.01),compared with the PDT 2.4 J group,the scratch healing rate and the number of invaded cells in the PDT 4.8 J group were significantly reduced(P<0.01).Compared with the PDT 2.4 J group,the PDT 4.8 J group had the expression levels of the three proteins is lower(P<0.01).Conclusion Photodynamic therapy can significantly inhibit the invasion and migration of melanoma cell lines A375 and B16-F10.The mechanism may be related to the inhibition of the expression of migration and invasion-related proteins.