Experimental Study on the Influence of Adenovirus-mediated Hepatocyte Growth Factor/vascular Endothelial Growth Factor 165 on the Survival and Mechanism of Hyper-proportion Random Flap in Rats
Objective To observe the effect of adenovirus-mediated hepatocyte growth factor/vascular endothelial growth factor 165(Ad-HGF/VEGF165)injection before flap operation on the survival and Mechanism of Hyper-proportion random flap in rats.Methods The model of Hyper-proportion random flap was designed on the back of rats and randomly divided into Ad-HGF/VEGF165 group,Ad-HGF/VEGF165 group and control group.Seven days before operation,Ad-HGF/VEGF165 group and Ad-HGF/VEGF165 group were separate multipoint injection Ad-HGF/VEGF165 and Ad-VEGF165 into the rat skin flap at multiple points in the experimental group,while 0.9%sodium chloride injection was injected in the control group.The survival area of the flap was observed on the 7 day and 14 day after operation,and the tissue samples of the flap were obtained for HE staining,the microvessel density and VEGF expression were observed by immunohistochemistry,and Western blot for detection of protein of target protein.Results On the 7 day and 14 day after operation,skin flap survival area,the number of microvessels,VEGF and the expression of extracellular regulated protein kinase(ERK1/2)protein in the Ad-HGF/VEGF165 group and Ad-HGF/VEGF165 group were higher than those in the control group(P＜0.001),and all indexes were significantly higher in the Ad-HGF/VEGF165 group than in the Ad-VEGF165 group(P＜0.05).Conclusion Ad-HGF/VEGF165 has a synergistic effect,which can significantly promote the expression of VEGF and ERK1/2 signal factors,increase the survival area of Hyper-proportion random flap,promote angiogenesis and improve the blood circulation of the flap.
hyper-proportion random flapgene-therapyadenovirusrevascularizationhepatocyte growth factorvascular endothelial growth factor
万方数据
维普
