Study on the Effect of Black and White Chinese Medicine on Melanin Metabolism by Regulating the Expression of MC1R and RTKs
Objective To investigate the effects of seven black Chinese herbs,such as Polygonum multiflorum Thunb,and Ligustrum lucidum,and seven white Chinese herbs,such as Bletilla striata and Angelica dahurica,on the expression of melanocortin 1 receptor(MC1R)and receptor tyrosine kinase(RTKs)in melanin synthesis of guinea pig skin.Methods The extract of traditional Chinese medicine was obtained by water extraction,and the skin tissue of the back of guinea pigs was obtained by gavage of the same amount in a 14 day cycle.The expression of MC1R protein and RTKs protein in the experimental and control groups were detected by immunohistochemistry and fluorescence methods.The results were statistically analyzed by Image J software.The effects of different traditional Chinese medicine environments on melanin synthesis in the skin tissue of guinea pigs were compared,and MC1R The relationship between RTKs protein and melanocyte metabolism.Results The white herbs with inhibitory effect on MC1R protein are Angelica dahurica,Poria cocos,Ampelopsis japonica,Atractylodes macrocephala,atractylodes macrocephala,among which the effects of Angelica dahurica,Atractylodes macrocephala,Atractylodes macrocephala are the most obvious,and the black herbs with significant inhibitory effect on MC1R are Ophiopogon flexuosus,Nigella sativa,Dangshen,Chickweed,among which the inhibitory effect of Dangshen is the most obvious.This result showed that the above 10 herbs had inhibitory effects on melanin synthesis,among which the inhibitory effects of Angelica dahurica,Cortex alba,Atractylodes macrocephala,and Salvia miltiorrhiza were the most obvious.Among them,the white herbs with inhibitory effect on RTKs protein are Angelica dahurica,Poria cocos,Ampelopsis pilosulae,Bupleurum officinale and Bupleurum officinale,among which Poria cocos,Ampelopsis pilosulae and Bupleurum officinale have the most obvious effect,and the black herbs with obvious effect are Nigella sativa and Salvia miltiorrhiza,among which Salvia miltiorrhiza has the most obvious effect.Conclusion There is a certain relationship between the color of medicinal materials and their efficacy.Both black and white drugs inhibit the action of MC1R and RTKs protein to varying degrees,and color image Chinese medicine may regulate α-MSH/M1CR and SCF/RTKs signal pathways affect melanocyte metabolism.
Color-effect phenomenon theory of traditional Chinese herbsblack and white Chinese herbsmelanocytesmelanocortin 1 receptorreceptor tyrosine kinase
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