Effect of Interference hsa_circ_0013958/miR-637 on the Proliferation,Migration and Invasion of Human Keloid Fibroblasts
Objective To explore the effect of circular RNA(circRNA)hsa_circ_0013958 on the proliferation,migration and invasion of human keloid fibroblasts and its molecular mechanism.Methods Human keloid fibroblasts HKF were divided into si-NC group,si-hsa_circ_0013958 group,miR-NC group,miR-637 group,si-hsa_circ_0013958+anti-miR-NC group,si-hsa_circ_0013958+anti-miR-637 group.Expression levels of hsa_circ_0013958 and miR-637 were detected by Real time quantitative polymerase chain reaction(RT-qPCR).Cell viability was assayed by Methyl thiazolyl tetrazolium(MTT)method,cell colony formation was detected by plate cloning test,cell migration and invasion ability was detected by transwell method,the targeting relationship between hsa_circ_0013958 and miR-637 was detected by dual luciferase reporter experiment.Results Compared with normal skin tissues,the expression level of hsa_circ_0013958 were elevated in keloid tissue,and the expression of miR-637 was decreased(P＜0.05).After interference with hsa_circ_0013958 expression or overexpression of miR-637,the survival rate,colony forming number,migration and invasion of HKF cells were decreased(P＜0.05).Hsa_circ_0013958 has a targeted regulatory relationship with miR-637,inhibition of miR-637 expression reversed the effect of interference with hsa_circ_0013958 on the proliferation,migration and invasion of HKF.Conclusion Interference with hsa_circ_0013958 may inhibit the proliferation,migration and invasion of human keloid fibroblasts by regulating miR-637.
国家科技期刊平台
NSTL
万方数据
维普
