Role Ferroptosis in the Inhibition of Proliferation and Osteogenic Differentiation of Periodontal Membrane Stem Cell under Inflammatory Microenvironment
Objective To study the role and mechanism of ferroptosis in the inhibition of proliferation and osteogenic differentiation of periodontal membrane stem cells(PDLSCs)under inflammatory microenvironment.Methods PDLSCs were cultured,cells treated with drug free medium served as control group,inflammation group was treated with medium containing 10 μg/L TNF-α,ferroptosis agonist group was treated with medium containing 10 μg/L TNF-α and 10 μmol/L erastin,and ferroptosis inhibitor group was treated with medium containing 10 μg/L TNF-α and 1 μmol/L ferrostatin-1.The expression levels of cell proliferation viability,transferrin receptor 1(TfR1),glutathione peroxidase(GPx4),the number of calcium nodules,the alkaline phosphatase(ALP)viability,the expression levels of Runt-related transcription factor 2(Runx 2),osteocalcin(OCN),and osteopontin(OPN)after osteogenic induction were measured.Results TfR 1 expression level of the inflammation group was higher than the control group,GPx4 expression level was lower the control group(P＜0.05).The proliferative activity of the inflammatory group at the 3rd,5th and 7th day,the level of calcium nodules OD540 at the 21st day of osteogenesis induction,and the expression levels of Runx2,OCN and OPN at the 7th day of osteogenesis induction were lower than those of the control group(P＜0.05).The above indexes in the iron death agonist group were lower than those in the inflammation group,and those in the iron death inhibitor group were higher than those in the inflammation group(P＜0.05).Conclusion The activation of ferroptosis in the inflammatory microenvironment suppresses PDLSCs proliferation and osteogenic differentiation.
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