自制中草药油对大鼠深度烧伤创面的疗效评价
Evaluation of Curative Effect of Self-made Chinese Herbal Oil on Deep Burn Wound in Rats
杜宪 1蔡霞 1任纪祯 1苏鲁安 2陈振雨1
作者信息
- 1. 青岛大学附属医院整形外科 山东 青岛 266000
- 2. 青岛君良烧伤医院烧伤外科 山东 青岛 266000
- 折叠
摘要
目的:经过建立Wister大鼠深度烧伤模型,探讨自制中草药油机制及促进深度烧伤创面愈合的临床疗效.方法:选取清洁健康雄性Wister大鼠60只为研究对象,将建模成功的大鼠依据治疗方式分为对照组30只和观察组30只.对照组使用生理盐水予以清创后,将凡士林纱布遮盖包裹创面,并用敷贴稳固;观察组采用自制中草药油涂抹,涂抹均匀后予以纱布进行固定,每日进行换药1次.对比两组治疗后第3、7、14、21天创面愈合情况与创面愈合率、HE染色法观察大鼠烧伤创面皮肤成纤维细胞数及毛细血管数,免疫组化染色法检测大鼠烧伤创面皮肤组织表皮生长因子(Epidermal growth factor,EGF)、血管内皮生长因子(Vascular endothelial growth factor,VEGF)水平.结果:治疗后第3、7、14、21天,两组创面愈合率均升高,且观察组明显高于对照组(P＜0.05);观察组创面愈合面积和渗出液均小于对照组;两组创面成纤维细胞数和毛细血管数均上升,且观察组明显高于对照组(P＜0.05);两组EGF、VEGF水平均上升,且观察组比对照组高(P＜0.05).结论:利用自制中草药油治疗大鼠深度烧伤创面,可有效增加创面成纤维细胞数和毛细血管数,提高创面EGF、VEGF水平,促进了创面愈合情况,为临床深度烧伤创面用药提供有力理论价值.
Abstract
Objective To explore the mechanism of self-made Chinese herbal oil and the clinical efficacy of promoting deep burn wound healing by establishing a Wister rat model of deep burn.Methods Sixty clean and healthy male Wister rats were selected for the study,and the modeled rats were divided into 30 control rats and 30 observation rats according to the treatment.After debridement with normal saline,the wound of control group was covered with vaseline gauze and fixed,the observation group was smeared with self-made Chinese herbal oil and fixed with Vaseline gauze after evenly smearing,the dressing was changed once every other day.The wound healing conditions and wound healing rates of the two groups at the 3rd,7th,14th and 21st days after treatment were compared,the number of fibroblasts and capillaries on the burn wound of rats was observed by HE staining method,the epidermal growth factor(EGF)and vascular endothelial growth factor(VEGF)levels on the burn wound of rats was detected by immunohistochemical staining method.Results On the 3rd,7th,14th and 21st day after treatment,the wound healing rate of the two groups was increased,and the observation group was significantly higher than the control group(P＜0.05),the wound healing area and exudate in the observation group were smaller than those in the control group.The number of fibroblasts and capillaries increased in the two groups,and the observation group was significantly higher than the control group(P＜0.05),the levels of EGF and VEGF were increased in the two groups,and the observation group was higher than the control group(P＜0.05).Conclusion The use of self-made Chinese herbal oil in the treatment of deep burn wounds in rats can effectively increase the number of fibroblasts and capillaries,improve the levels of EGF and VEGF,and promote the healing of wounds.
关键词
自制中草药油/大鼠/深度烧伤/创面愈合/疗效评价Key words
self-made Chinese herbal oil/rat/deep burn/wound healing/efficacy evaluation引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据