The Role and Mechanism of 2-APB Inhibits the Apoptosis of Condylar Chondrocytes Induced by Static Pressure
Objective To explore the inhibitory effect and mechanism of TRPM7 inhibitor 2-APB on static pressure induced by apoptosis of condylar chondrocytes.Methods The rat condylar chondrocytes were extracted through trypsin digestion method.Different static pressure conditions were administered.After the 2-APB intervention,intracellular calcium concentration was detected by Flou-3AM fluorescence probe.The apoptosis rate was detected by flow cytometry.The expression of p38 protein was detected by Western Blot,and the expression of p38mRNA was detected by RT-PCR.p38 inhibitor was further used to intervene and analyze the changes of cell apoptosis.Results Under different pressure conditions,the change trend of intracellular calcium concentration was highly consistent with that of cell apoptosis rate.The Pearson correlation value was 0.916(P＜0.01),showing a significant positive correlation.2-APB significantly inhibited the increase of intracellular calcium concentration induced by static pressure(P＜0.01).There was no significant difference between 2-APB and BAPTA-AM in inhibiting intracellular calcium concentration(P＞0.05).2-APB could significantly inhibit the increase of cell apoptosis rate and the high expression of p38 protein and mRNA induced by static pressure(P＜0.01).SB203580 could significantly inhibit the increase of cell apoptosis rate induced by static pressure(P＜0.01).Conclusion The increase of calcium ion mediated by TRPM7 under static pressure is the way to cause cell apoptosis injury,and p38 plays an important role in it.Inhibiting TRPM7 mediated calcium ion influx may be a way to alleviate cell apoptosis induced by static pressure.
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