Effects of Natural Component of Zanthoxylum Xanthoxylum(WGX-50)on Moisture Retention and Skin Barrier Function
Objective To study the moisturizing properties of the natural component of Zanthoxylum WGX-50 and its effect on skin barrier function.Methods Keratinocyte HaCaT and 16 subjects with good skin condition were studied.HaCaT cells were used to test the cytotoxicity of WGX-50,and the effects of WGX-50 on the expression levels of aquaporin 3(AQP3),Filaggrin,Claudin-1,hyaluronic acid synthetase 2(HAS2)and CD44 were analyzed.Subjects were used to test the effects of WGX-50 on skin barrier function.Results 0.5 μmol/L WGX-50 promoted HaCaT cell proliferation(P＜0.05),and 1.0-100.0 μmol/L WGX-50 inhibited HaCaT cell activity,and the cell activity was lower than that of control group(P＜0.05).In particular,high concentration of WGX-50(≥10 μmol/L)significantly inhibited the activity of HaCaT cells(P＜0.01).0.5 μmol/L WGX-50 could effectively promote AQP3 gene expression(P＜0.05),but had no significant effect on Filaggrin,Claudin-1,HAS2 and CD44 gene expression levels(P＞0.05).Experiments on skin moisture content and transepidermal moisture loss rate showed that xanthoxylin WGX-50 had good moisturizing effect on skin.Conclusion Low concentration of xanthoxylin(0.5μmol/L)WGX-50 can promote the proliferation of HaCaT cells,enhance the expression of AQP3 gene in HaCaT cells,and improve the function of human skin barrier.
xanthoxylinskin barrier functionmoisturizewater contentepidermal water loss rate
万方数据
维普
