首页|Two distinct subpopulations of marginal zone B cells exhibit differential antibody-producing capacities and radioresistance
Two distinct subpopulations of marginal zone B cells exhibit differential antibody-producing capacities and radioresistance
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Marginal zone(MZ)B cells,which are splenic innate-like B cells that rapidly secrete antibodies(Abs)against blood-borne pathogens,are composed of heterogeneous subpopulations.Here,we showed that MZ B cells can be divided into two distinct subpopulations according to their CD80 expression levels.CD80high MZ B cells exhibited greater Ab-producing,proliferative,and IL-10-secreting capacities than did CD80low MZ B cells.Notably,CD80high MZ B cells survived 2-Gy whole-body irradiation,whereas CD80low MZ B cells were depleted by irradiation and then repleted with one month after irradiation.Depletion of CD80low MZ B cells led to accelerated development of type Ⅱ collagen(CⅡ)-induced arthritis upon immunization with bovine CⅡ.CD80high MZ B cells exhibited higher expression of genes involved in proliferation,plasma cell differentiation,and the antioxidant response.CD80high MZ B cells expressed more autoreactive B cell receptors(BCRs)that recognized double-stranded DNA or CⅡ,expressed more immunoglobulin heavy chain sequences with shorter complementarity-determining region 3 sequences,and included more clonotypes with no N-nucleotides or with B-1a BCR sequences than CD80low MZ B cells.Adoptive transfer experiments showed that CD21+CD23+transitional 2 MZ precursors preferentially generated CD80low MZ B cells and that a proportion of CD80low MZ B cells were converted into CD80high MZ B cells;in contrast,CD80high MZ B cells stably remained CD80high MZ B cells.In summary,MZ B cells can be divided into two subpopulations according to their CD80 expression levels,Ab-producing capacity,radioresistance,and autoreactivity,and these findings may suggest a hierarchical composition of MZ B cells with differential stability and BCR specifiicity.
Marginal Zone B CellCD80AutoreactivityRadioresistance
Sujin Lee、Yeunjung Ko、Hyun Woo Lee、Won Joon Oh、Hun Gi Hong、Dinuka Ariyaratne、Se Jin Im、Tae Jin Kim
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Department of Immunology,Graduate School of Basic Medical Science,Sungkyunkwan University School of Medicine,Suwon 16419,Republic of Korea
Immunology and Microbiology Graduate Program,Baylor College of Medicine,Houston,TX 77030,USA
Department of Immunology and Molecular Medicine,Faculty of Medical Sciences,University of Sri Jayewardenepura,Nugegoda,Sri Lanka
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