Determination of Soluble Sugar in Sisal Leaf by HPLC
Different concentration ethanol extracting solutions were selected to study the extraction efficiency for glucose,fructose and sucrose.The high efficiency HPLC way of measuring the three soluble sugars simultaneously was expected to be constructed.The results showed that 60% ethanol was the optimal extraction solution,with relative standard deviations of 1.0% ~4.6%,association coefficients above 0.999 and the adding standard recoveries of 98.5% ~99.4%.The total soluble sugar contents were up to 167 mg/g,in which the glucose contents were the highest with the dry weight of 74 mg/g.