Expression Pattern and Bioinformatics Analysis of miR156 Gene Family of Industrial Hemp Under NaHCO3 Stress
The miR156 gene family of industrial hemp plays an important role in the regulation of re-sponse to alkaline salt stress.In order to explore the molecular mechanism of miR156 gene family of in-dustrial hemp in response to NaHCO3 stress,this gene family of Huoma No.1(aline-alkali tolerant varie-ty)was analyzed by bioinformatics and qRT-PCR.The results showed that the miR156 family gene of in-dustrial hemp is closely related to asparagus,wild strawberry,apple and soybean.The mature miR156,derived from the two pre-miR156 sequences,exhibited higher conservation.During the 24 hours of NaH-CO3 stress,the expression of the miR156 gene in industrial hemp initially increased,followed by a de-crease and subsequent increase as the stress duration prolonged.The findings of this study established a theoretical foundation for further exploration into the regulatory network of the miR156 gene family and its target genes in industrial hemp under saline-alkali stress.
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