The miR156 gene family of industrial hemp plays an important role in the regulation of re-sponse to alkaline salt stress.In order to explore the molecular mechanism of miR156 gene family of in-dustrial hemp in response to NaHCO3 stress,this gene family of Huoma No.1(aline-alkali tolerant varie-ty)was analyzed by bioinformatics and qRT-PCR.The results showed that the miR156 family gene of in-dustrial hemp is closely related to asparagus,wild strawberry,apple and soybean.The mature miR156,derived from the two pre-miR156 sequences,exhibited higher conservation.During the 24 hours of NaH-CO3 stress,the expression of the miR156 gene in industrial hemp initially increased,followed by a de-crease and subsequent increase as the stress duration prolonged.The findings of this study established a theoretical foundation for further exploration into the regulatory network of the miR156 gene family and its target genes in industrial hemp under saline-alkali stress.
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