用于工业大麻种质资源筛选和鉴定的KASP标记集开发
Development of KASP Marker Set for Screening and Identification of Industrial Hemp Germplasm Resources
周佳 1张园 2郭孟璧 2吕品 2杜光辉 3许艳萍 2杨明 2张庆滢 2字雪靖 2陈璇2
作者信息
- 1. 云南大学农学院,云南 昆明 650504;云南省农业科学院经济作物研究所,云南 昆明 650205
- 2. 云南省农业科学院经济作物研究所,云南 昆明 650205
- 3. 云南大学农学院,云南 昆明 650504
- 折叠
摘要
目前,我国工业大麻种质资源的分子筛选和分子鉴定主要依赖于AFLP、SSR和重测序等方法,缺乏快速、准确、直观的分子标记体系,开发竞争性等位基因特异性PCR(Kompetitive Al-lele Specific PCR,KASP)标记集及其检测体系具有良好的应用前景.研究首先基于大麻素B位点基因多态性成功开发了1 个特异性KASP位点,能够准确区分大麻种质资源中的低毒型、高毒型和中间型三种化学型;随后基于来自95 份不同大麻种质资源的全基因组重测序SNP 数据,总共开发了32 个高质量的核心KASP 标记.验证表明,该 33 个KASP 标记集及其检测体系可以应用于工业大麻辅助育种、工业大麻品种分子鉴别和指纹图库构建等,丰富了工业大麻分子标记类型和方法.
Abstract
Currently,the molecular screening and identification of industrial hemp germplasm re-sources in China mainly rely on methods such as AFLP,SSR and Resequencing,lacking in a fast,accu-rate,and intuitive molecular marker system.The development of Kompetitive Allele Specific PCR(KASP)with marker sets and its detection system has good application prospects.This study first suc-cessfully developed a specific KASP locus based on the cannabinoid B locus gene polymorphism,which can accurately distinguish different chemical types of low-THC,high-THC or intermediate types in can-nabis germplasm resources.A total of 32 high-quality core KASP markers were developed based on the whole-genome resequencing single nucleotide polymorphism(SNP)data from 95 different cannabis germ-plasm resources.Verification experiment showed that the 33 KASP marker sets and its detection system can be applied to industrial hemp assisted breeding,molecular identification of industrial hemp varieties,and fingerprint library construction,enriching the types and methods of industrial hemp molecular mark-ers.
关键词
工业大麻/种质鉴定/大麻素/KASP标记/指纹图谱Key words
industrial hemp/germplasm identification/cannabinoid/KASP marker/fingerprint引用本文复制引用
基金项目
财政部和农业农村部:国家麻类产业技术体系建设项目育种技术与方法岗位项目(CARS-16-E02)
云南省科技计划(202305AP350011)
云南省高层次人才培养支持计划(青年拔尖)()
出版年
2024
国家科技期刊平台
NSTL
万方数据