工业大麻CsMYB17基因克隆与表达分析
Cloning and Expression Analysis of CsMYB17in Industrial Hemp
周智满 1许英 1程超华 1戴志刚 1张小雨 1杨泽茂 1陈基权 1邓灿辉 1粟建光 1唐蜻1
作者信息
- 1. 中国农业科学院麻类研究所,湖南 长沙 410221
- 折叠
摘要
为探究CsMYB17基因在工业大麻发育过程中的潜在功能,试验以工业大麻DMG265为材料,对CsMYB17基因进行克隆,并对其编码蛋白进行生信分析.结果表明,CsMYB1基因的CDS区为1005 bp,编码334 个氨基酸,属于亲水性蛋白,并且无跨膜结构和信号肽,其二级结构中无规则卷曲占比最高,与青蒿AaMYB同源性较高.对CsMYB17在大麻不同组织中的表达模式进行分析,结果表明,CsMYB17在雌花、腺毛中表达量相对较高,研究结果为了解CsMYB17在工业大麻发育中的功能提供了理论基础.
Abstract
To explore the potential function of CsMYB17 gene in the development of industrial hemp,CsMYB17was cloned from accession DMG265,and its sequence was used for bioinformatics a-nalysis.The results showed that CDS of CsMYB17region was 1005 bp,encoding 334 amino acids,which belonged to hydrophilic proteins and had non-transmembrane structure and signal peptide.The secondary structure of CsMYB17was random coil,which had a high homology with AaMYB of Artemisia annua.The expression pattern of CsMYB17in different tissues was analysed.The results showed that the expres-sion of CsMYB17was higher in female flowers and glandular trichomes than in other tissues.The study provides a theoretical basis for understanding the function of CsMYB17in the development of industrial hemp.
关键词
工业大麻/CsMYB17/基因克隆/表达分析Key words
industrial hemp/CsMYB17/gene cloning/expression analysis引用本文复制引用
基金项目
财政部和农业农村部:国家麻类产业技术体系建设专项(CARS-16-E01)
中国农业科学院创新工程项目(CAAS-ASTIP-2017-IBFC01)
出版年
2024
国家科技期刊平台
NSTL
万方数据