Cloning and Analysis of the Acid 12-Hydroxylase Promoter in Castor
Ricinus communis L.is an important industrial oil crop,and the main component of castor oil is ricinoleic acid.Oleate 12-hydroxylase(FAH12)is a key enzyme in the regulation of ricinoleic acid biosynthesis,which is efficiently expressed only in the seeds of the castor bean,displaying characteristic tissue-specific expression.It has been found that the regulatory elements of FAH12gene expression are mainly concentrated in the region from-1 bp to-1432 bp(24680984～24682416)upstream of the gene,including 84 TATA core promoter elements,42 standard promoter regulatory elements CAAT-Box,and 2 specific expression elements Skn-1 related to endosperm formation.Using the castor bean genome as the material,the promoter of the ricinoleic acid 12-hydroxylase gene was obtained and inserted into the pCAMBIA1305.2 expression vector,replacing the constitutive promoter CaMV35S,and named as pCAMBIA-FA12P.The expression vector pCAMBIA-FA12P constructed in this study can be used for subsequent studies on guiding the specific expression of target genes in castor bean.
万方数据
维普
