Comparison and Identification of Tetraploid Induction Methods of Kenaf(Hibiscus cannabinus L.)
This study aimed to obtain tetraploid Hibiscus cannabinus L.(4n=4x=72)plants by trea-ting diploid Hibiscus cannabinus L.(2n=2x=36)seeds and seedling apical meristems with different con-centrations of colchicine.Variations were observed and identified through stomatal examination,flow cy-tometry,and chromosome counting.Results indicated that the immersion method with 0.2%colchicine concentration for 24 hours exhibited superior induction efficacy,with a variation rate of 53.85%and a survival rate of 25.49%.Conversely,the cotton ball covering method with 0.4%colchicine concentration for 5 days yielded a lower induction efficacy with a variation rate of 15%.This study has successfully de-veloped novel germplasm of kenaf,providing valuable references for the breeding of kenaf polyploids.
万方数据
维普
