Isolation and Identification of Fusarium wilt Pathogen from Flax in Xinjiang Uygur Autonomous Region and Fungicides Screening
The pathogen of flax wilt disease from the diseased plants collected from the Ili Kazakh Autonomous Prefecture of Xinjiang Uygur Autonomous Region was isolated in this study.The pathogen was identified as Fusarium oxysporum through colony morphology identification,spore detection,ITS de-tection,and Koch's postulates.The effects of Fusarium oxysporum on the germination rate and seedling growth of flax seeds were determined.The results showed that Fusarium oxysporum inhibited the germina-tion rate of flax seeds and the growth of flax seedlings.Twelve fungicides were selected to determine the toxicity of Fusarium oxysporum in laboratory.The results showed that the lowest EC50 value was 98%Car-bendazim with 1.2002 mg/L,followed by 97%Prochloraz with 6.0109 mg/L.The highest EC50 value was 97.1%Azoxystrobin with 1080.7229 mg/L,while the EC50 value of the Biopesticide——200 million CFU/mL Rhodopseudomonas palustris PSB-S Suspension Concentrate(SC)was 7.3919×105 CFU/mL.To sum-marize,it can be seen that Carbendazim,Prochloraz,and 2×108 CFU/mL PSB-S SC can be used as al-ternative fungicides for field application to control flax wilt disease.
flax wilt diseaseisolation and identificationscreening of fungicides