首页|果胶酶苎麻脱胶:一种生产还原糖的新途径

果胶酶苎麻脱胶:一种生产还原糖的新途径

扫码查看
为提高苎麻生物质利用效率,使用果胶酶对苎麻韧皮进行水解,通过Box-Behnken响应面法优化果胶酶水解苎麻果胶工艺,采用3,5-二硝基水杨酸(DNS)法检测果胶水解液中的还原糖含量.结果表明:还原糖最佳提取工艺为水解温度52℃,果胶酶浓度4.8%,水解时间7h.在该条件下,水解液中还原糖含量可达(10.32±0.47)mg/mL.该工艺适用于将苎麻韧皮中果胶组分转化为可溶性还原糖,为后续生物质资源再利用提供依据.
Degumming Ramie by Pectinase:a New Way to Produce Reducing Sugar
To improve the utilisation efficiency of ramie biomass,this study uses pectinase to hydrol-yse ramie bast.The Box-Behnken response surface methodology was used to optimise the pectinase hy-drolysis process of ramie pectin.The reducing sugar content in the pectin hydrolysate was determined by using the 3,5-dinitrosalicylic acid(DNS)method.The optimal process for extracting the reducing sugar content was found to be a hydrolysis temperature of 52℃,a pectinase concentration of 4.8%,and a hy-drolysis time of 7 h.Under these conditions,the reducing sugar content of the hydrolysate could reach(10.32±0.47)mg/mL.This process is technically reliable and suitable for converting ramie pectin into reducing sugar and provides a basis for the subsequent reuse of biomass resources.

ramiepectinresponse surface methodologyreducing sugar content

马世航、成莉凤、冯湘沅、杨琦、席果果、彭正红、段盛文

展开 >

中国农业科学院麻类研究所,湖南 长沙 410221

苎麻 果胶 响应面法 还原糖含量

中国农业科学院科技创新工程项目财政部和农业农村部:国家麻类产业技术体系资助国家自然科学基金湖南省自然科学基金项目湖南省自然科学基金项目所级"岳麓青年人才"项目长沙市自然科学基金项目长沙市自然科学基金项目新疆维吾尔自治区区域协同创新专项(科技援疆计划)项目

ASTIP-IBFC-05CARS-16-E22323012812023JJ503152023JJ30621IBFC-YLQN-202102kq2208250kq22082472022E2105

2024

中国麻业科学
中国农业科学院麻类研究所

中国麻业科学

CSTPCD
影响因子:0.492
ISSN:1671-3532
年,卷(期):2024.46(5)