HPLC法测定蒙药材金露梅中没食子酸含量
Determination of Gallic Acid in Mongolian Medicine Potentilla Fruticosa by HPLC
郭凤英 1闫凤杰 1关金凤 1莫日根高娃 1于海云1
作者信息
- 1. 通辽市市场检验检测中心,内蒙古 通辽 028000
- 折叠
摘要
目的:建立高效液相法测定金露梅中没食子酸含量.方法:采用Kromasil 100-5-C18(250 mm×4.6 mm,5 μm)色谱柱,以甲醇-0.1％磷酸(10∶90)为流动相,流速为1.0 mL/min,检测波长273 nm,柱温30℃,进样量为10μL.结果:没食子酸在3.44～137.52 μg/mL范围内线性关系良好,相关系数r=1.000;平均回收率为99.4％(RSD％=1.588％);6批不同产地金露梅中没食子酸含量在3.156～11.537 mg/g之间.结论:所建立的高效液相法稳定可靠,可用于金露梅中没食子酸的含量测定.
Abstract
Objective To establish an high performance liquid chromatography method for determination of gallic acid in Mongolian medicine potentilla fruticosa.Methods The test was performed in Kromasil 100-5-C18(250 mm ×4.6 mm,5 µm)under the elu-tion of methanol-0.1％phosphoric acid(10:90).The flow rate was 1.0 ml/min and the wavelength was set as 273 nm.The col-umn temperature was 30 ℃ and the injection volume was 10 μL.Result The liner ranges of gallic acid fell into 3.44-137.52 μg/mL(r=1.000).The average recovery of gallic acid was 99.4％(RSD=1.588％).The determination of galllic acid in potentilla fru-ticosa from 6 places of origin fell into 3.156-11.537 mg/g.Conclusion The established method is simple,stable and reliable.It can be used for determination of gallic acid in Mongolian medicine potentilla fruticosa.
关键词
蒙药材/金露梅/没食子酸/高效液相/含量测定Key words
Mongolian Medicine/Potentilla Fruticosa/Gallic Acid/HPLC/Contention Determination引用本文复制引用
出版年
2024
NETL
NSTL
维普
万方数据