The Effect of Shui People Qubi Formula on the Ultrasonic Performance,the Pathological Evaluation,the Expression Level of MMP-3 and Collagen Ⅱ in KOA Rabbits
Objective To study the effect of Shui People Qubi Formula on the ultrasonic performance,the pathological evaluation,the expression level of MMP-3 and Collagen Ⅱ in KOA rabbits.Methods 30 white rabbits were randomly divided into normal group,model group,and Shui People Qubi Formula treatment group,10 rabbits in each group.Knee osteoarthritis model was established by intra-articular injection of papain.The rabbits were treated twice a day.28 days after treatment,the joint characteristic of each group was evaluated by ultrasound and pathological detection separately,and MMP-3 and Collagen Ⅱ expression level in articular cartilage were detected by immunohistochemistry.Results Compared with the normal group,the Ridit value and pathological Mankins score of the model group rabbits significantly increased(P＜0.05),MMP-3 expression in knee articular cartilage significantly increased(P＜0.05),and Collagen Ⅱ expression significantly decreased(P＜0.05).Compared with the model group,the R value and pathologi-cal Mankins score of rabbits in the Shui People Qubi Formula treatment group significantly decreased(P＜0.05),MMP-3 expression in knee articular cartilage significantly decreased(P＜0.05),and Collagen Ⅱ expression significantly increased(P＜0.05).Conclu-sion Shui People Qubi Formula can reduce the degeneration of cartilage matrix causing by MMP-3,it also can alleviate synovial prolif-eration and reduce knee joint angiogenesis,which is to delay joint degeneration.
Shui People Qubi FormulaKnee OsteoarthritisMMP-3Collagen Ⅱ
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