Research on Optimization of Total Flavonoid and Polyphenol Extraction Process of Elaeagnus moorcroftii Wall.Ex Schlecht.Based on Response Surface Method
Objective Combined with the single-factor test,the Box-Behnken design principle was used to design the response surface method to optimize the extraction process of total flavonoids and polyphenols from Elaeagnus Moorcroftii Wall.ex Schlecht.Methods On the basis of the single-factor test,the extraction time,liquid-material ratio and ethanol concentration were taken as the flavonoid investigation factors,and the extraction time,liquid-material ratio and acetone volume fraction were taken as the poly-phenol investigation factors,and the response surface method was used to optimize the extraction process conditions.Results The opti-mal extraction conditions of total flavonoids:when the extraction time was 28.7 min,the liquid-material ratio was 17.25∶1,and the ethanol concentration was 57.6％,the extraction rate of total flavonoids reached the maximum value of 4.15％.The optimal extraction conditions of total polyphenols:when the extraction time was 40.55 min,the liquid-material ratio was 10.32∶1,and the volume frac-tion of acetone was 51.1％,the extraction rate of total polyphenols reached the maximum value,which was 1.19％.Conclusion The extraction of total flavonoids and polyphenols from Elaeagnus Moorcroftii Wall.ex Schlecht is reasonable and reproducible,which can provide a theoretical basis for the in-depth development of Elaeagnus Moorcroftii Wall.ex Schlecht.
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