大孔树脂纯化蒙自藜芦总生物碱提取工艺研究
Study on the Purification Technology of Total Alkaloids from the Veratrum mengtzeanum with Macroporous Resin
吴倩 1侯安国 2朱常成 3尹子丽1
作者信息
- 1. 云南中医药大学云南省傣医药与彝医药重点实验室,云南 昆明 650000
- 2. 云南中医药大学中药学院,云南 昆明 650000
- 3. 云南自由贸易试验区睿之成医药科技有限公司,云南 昆明 650217
- 折叠
摘要
目的:对蒙自藜芦总生物碱提取工艺进行优化.方法:以蒙自藜芦总生物碱含量及3个成分藜芦碱、藜芦胺、介芬胺为指标,通过静态吸附和动态吸附实验考查上样体积、上样液质量浓度、径高比、上样液PH值、水洗用量、洗脱剂及其用量,优化出最佳纯化工艺条件.结果:选用D101型大孔树脂纯化蒙自藜芦总生物碱提取物,上样体积50 mL,径高比1∶5,上样液总生物碱质量浓度为0.2 mg/mL,上样液pH值为9,水洗用量为4 BV,洗脱剂为80％乙醇,洗脱剂用量为8 BV,纯化后的蒙自藜芦总生物碱转移率大于60％,含量为62.88％,比纯化前提高5.74倍.结论:D101型大孔树脂纯化蒙自藜芦总生物碱工艺稳定可行,可为后续制剂开发提供参考依据.
Abstract
Objective In Veratrum mengtzeanum total alkaloid extraction technology was optimized.Methods Taking the total al-kaloid content and three components veratrine,veratrine,and mesofenamine as indicators,the sample volume,mass concentration of sample solution,diameter/height ratio,pH value of sample solution,amount of water washing,eluent and its amount of sample solu-tion were examined by static adsorption and dynamic adsorption experiments,and the best purification process conditions were opti-mized.Results D101 macroporous resin was used to purify the total alkaloid extract of Veratrum mengtzeanum.The sample volume was 50mL and the diameter/height ratio was 1∶5,on the quality of samples,total alkaloids concentration is 0.2 mg/mL,pH value of the sample fluid 9,water dosage of 4 bv,eluent is 80％ethanol,dosage of eluent is 8 bv,purified of Veratrum mengtzeanum transfer rate more than 60％of total alkaloid content is 62.88％,and 5.74 times higher than that of before purification.Conclusion D101 macro-porous resin purification process is stable and feasible,which can provide a reference for the subsequent preparation development.
关键词
蒙自藜芦/总物碱/大孔树脂/纯化工艺Key words
Veratrum mengtzeanum/Total Alkaloids/Macroporous Resin/Purification Technology引用本文复制引用
出版年
2025
NETL
NSTL
万方数据