Study on the Purification Technology of Total Alkaloids from the Veratrum mengtzeanum with Macroporous Resin
Objective In Veratrum mengtzeanum total alkaloid extraction technology was optimized.Methods Taking the total al-kaloid content and three components veratrine,veratrine,and mesofenamine as indicators,the sample volume,mass concentration of sample solution,diameter/height ratio,pH value of sample solution,amount of water washing,eluent and its amount of sample solu-tion were examined by static adsorption and dynamic adsorption experiments,and the best purification process conditions were opti-mized.Results D101 macroporous resin was used to purify the total alkaloid extract of Veratrum mengtzeanum.The sample volume was 50mL and the diameter/height ratio was 1∶5,on the quality of samples,total alkaloids concentration is 0.2 mg/mL,pH value of the sample fluid 9,water dosage of 4 bv,eluent is 80％ethanol,dosage of eluent is 8 bv,purified of Veratrum mengtzeanum transfer rate more than 60％of total alkaloid content is 62.88％,and 5.74 times higher than that of before purification.Conclusion D101 macro-porous resin purification process is stable and feasible,which can provide a reference for the subsequent preparation development.
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