Comparison of efficiencies of chemiluminescence immunoassay and enzyme-linked immunosorbent assay in diagnosis of viral hepatitis type B
Objective:To compare efficiencies of chemiluminescence immunoassay(CLIA)and enzyme-linked immunosorbent assay(ELISA)in diagnosis of viral hepatitis type B(hepatitis B).Methods:A total of 154 patients with suspected hepatitis B admitted to the hospital from May 2020 to May 2022 were selected as the research objects.The levels of hepatitis B serum markers[hepatitis B surface antigen(HBsAg),hepatitis B surface antibody(HBsAb),hepatitis B e antigen(HBeAg),hepatitis B e antibody(HBeAb),hepatitis B core antibody(HBcAb)]were detected by CLIA and ELISA.Using the results of fluorescence quantitative polymerase chain reaction(PCR)as the gold standard,the diagnostic efficiencies of CLIA and ELISA for hepatitis B and the detection rate of low-level HBsAg were compared.Results:Among the 154 suspected hepatitis B patients,92 cases were positive and 62 cases were negative by fluorescence quantitative PCR;the HBsAg levels were 0.06-0.10 U/mL in 5 cases,0.11-0.50 U/mL in 5 cases,0.51-2.50 U/mL in 8 cases,and 2.51-3.00 U/mL in 2 cases.There were 89 positive cases and 65 negative cases diagnosed by CLIA.There were 78 cases were positive and 76 cases were negative by ELISA.The sensitivity,the accuracy and the negative predictive value of CLIA in the diagnosis of hepatitis B were higher than those of ELISA,the missed diagnosis rate was lower than that of ELISA,and the differences were statistically significant(P<0.05).The detection rate of HBsAg level of 0.06-0.10 U/mL by CLIA method was higher than that by ELISA method,and the difference was statistically significant(P<0.05).Conclusions:The diagnostic efficiency of CLIA in hepatitis B is higher than that of ELISA.
Chemiluminescence immunoassayEnzyme-linked immunosorbent assayViral hepatitis type BSerological markerEfficiency
万方数据
维普
