Efficiency of SAT-TB combined with FQ-PCR in diagnosis of sputum smear-negative pulmonary tuberculosis
Objective:To analyze efficiency of RNA simultaneous amplification and testing for Mycobacterium tuberculosis(SAT-TB)combined with fluorescence quantitative polymerase chain reaction(FQ-PCR)in diagnosis of sputum smear-negative pulmonary tuberculosis patients.Methods:A prospective study was conducted on 75 patients with suspected sputum smear-negative pulmonary tuberculosis admitted to the hospital from July 2020 to July 2022.The bronchoalveolar lavage fluid samples were collected from all patients and detected by SAT-TB and FQ-PCR.Using the results of Mycobacterium tuberculosis sputum culture as the'gold standard',the SAT-TB,single FQ-PCR single and combined detection in the diagnosis value of sputum smear-negative pulmonary tuberculosis were compared.Results:The gold standard showed that among the 75 patients with suspected sputum smear-negative pulmonary tuberculosis,48 cases were positive and 27 cases were negative;SAT-TB detection showed that 36 cases were positive and 39 cases were negative;FQ-PCR detection showed that 37 cases were positive and 38 cases were negative;and SAT-TB combined with FQ-PCR detection showed that 47 cases were positive and 28 cases were negative.The sensitivity,the accuracy of SAT-TB combined with FQ-PCR detection in the diagnosis of sputum smear-negative pulmonary tuberculosis were higher than those of SAT-TB and FQ-PCR single detection,the missed diagnosis rate was lower than that of SAT-TB and FQ-PCR single detection,and the differences were statistically significant(P<0.05).Conclusions:The efficiency of SAT-TB combined with FQ-PCR in the diagnosis of sputum smear-negative pulmonary tuberculosis is higher than that of the two single tests.
RNA simultaneous amplification and testing for Mycobacterium tuberculosisFluorescence quantitative PCRSputum smear negativePulmonary tuberculosisAccuracy
NETL
NSTL
万方数据
维普
