Diagnostic efficiency of combined detection of serum IL-18,IL-22 and CD4+CD25+CD127low Treg levels in patients with active lupus nephritis
Objective:To analyze diagnostic efficiency of combined detection of serum interleukin-18 (IL-18),interleukin-22 (IL-22) and CD4+CD25+CD127low regulatory T cells (Treg) levels in patients with active lupus nephritis. Methods:The clinical data of 64 patients with lupus nephritis admitted to this hospital from January 2022 to September 2023 were retrospectively analyzed and they were set as the study group. According to the severity of the disease,they were divided into active and stable patients. Another 64 healthy subjects in the same period were selected as the control group. The levels of serum IL-18,IL-22,CD4+CD25+CD127low Treg were compared between the two groups and among the patients with different severity of disease,and their correlations with the severity of lupus nephritis were analyzed. The receiver operating characteristic (ROC) curve was used to analyze the diagnostic efficiencies of single and combined detection of each index level in the patients with active lupus nephritis. Results:The levels of serum IL-18 and IL-22 in the study group were higher than those in the control group,the levels of CD4+CD25+CD127low Treg were lower than those in the control group,and the differences were statistically significant (P<0.05). The levels of serum IL-18 and IL-22 in the patients in active stage were higher than those in the patients in stable stage,the level of CD4+CD25+CD127low Treg was lower than that in the patients in stable stage,and the differences were statistically significant (P<0.05). Pearson correlation analysis showed that the serum IL-18 and IL-22 levels were positively correlated with the severity of lupus nephritis (r>0,P<0.05),and CD4+CD25+CD127low Treg levels were negatively correlated with the severity of lupus nephritis (r<0,P<0.05). ROC curve analysis showed that the areas under the curve of single and combined detection of serum IL-18,IL-22,CD4+CD25+CD127low Treg levels for the diagnosis of active lupus nephritis were 0.828,0.817,0.788 and 0.921,separately;and the diagnostic efficiency of combined detection was higher than that of single detection. Conclusions:The combined detection of IL-18,IL-22 and CD4+CD25+CD127low Treg levels is more effective than the single detection of the three in the diagnosis of active lupus nephritis.
Interleukin-18Interleukin-22CD4+CD25+CD127low regulatory T cellLupus nephritisActive stage