Study on HPLC Fingerprint and Content Determination of Two Components of Mongolian Medicine Guangmingyan Siwei Tangsan
Objective:To establish the HPLC fingerprint of Guangmingyan Siwei Tangsan,determine the content of Gallic acid and Piperine,and improve the quality standard of Guangmingyan Siwei Tangsan.Method:HPLC method was used,with Agilent 5 TC-C18(4.6 mm× 250 mm)as the chromatographic column;The mobile phase is 0.1％phosphoric acid water(A)-methanol(B)gradient elution;The flow rate is 1 mL·min-1;The UV detection wavelength is 300 nm;The column temperature is 30 ℃;Injection volume is 10 μ L.Result:A total of 11 common peaks were identified in the HPLC plots of 10 batches of samples,with a calculated similarity of over 0.995.The No.3 peak is Gallic acid,and the No.10 peak is Piperine.Gallic acid in 21.76 μ g·mL-1 -130.56 μ g·mL-1 range,There is a good linear relationship,r=0.9997;Piperine in 105.1 μ g·mL-1-420.4 μ g·mL-1 range,There is a good linear relationship,r=0.9990;The average sample recovery rates were 98.73％and 99.26％,respectively,with RSD less than 2％.Conclusion:This method is simple and accurate,and can be used for the quality control of Guangmingyan Siwei Tangsan,improving the quality standard of the drug.
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