Objective:To investigate the effects of Qi Mai Yi Xin granule(QMG)on cardiac function and myocardial tissue apoptosis in rats with acute myocardial ischemia induced by left anterior descending coronary artery(lad)ligation,and to provide evidence for its clinical application.Methods:Wistar rats were randomly divided into control group,model group,QMG Group and Isosorbide Mononitrate group.The Rats in the control group and the model group were treated with distilled water,the rats in the QMG group were treated with herbal medicine 2.43 g KG·d and the rats in the Isosorbide Mononitrate group were treated with herbal medicine 1mg KG·d.Except the control group,the rats in the other groups were made by ligating the left anterior descending coronary artery.The concentrations of CPK and LDH in serum were observed.The expression of TNF-α and Fas mRNA in myocardium was detected by RT-PCR,and the expression of FAS and Caspase-3 in myocardium was detected by Western blot.Results:qmg could decrease the concentration of CPK and LDH,down-regulate the expression of TNF α and Fas,down-regulate the expression of FAS and caspase 3.Conclusion:QMG has a protective effect on myocardium of rats with acute myocardial ischemia,and its mechanism may be related to down-regulation of death pathway receptor and ligand to antagonize myocardial apoptosis.
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