Objective:To observe the improvement effect of Gamuzhuer enema on colonic lesions of colitis model rats,and to study its effect on IL-6/STAT3 signaling pathway.Methods:40 rats were divided at random into 4 groups:blank,model,sulfasalazine and Gamuzhuer,with 10 in each group.After the model was made by ethanol/TNBS enema;normal saline,sulfasalazine and Gamuzhuer enema were used respectively.After 14 days,the colon was taken for observation with naked eye and microscope.Immunohistochemical analysis was performed to evaluate the expression of IL-6 and STAT3 in colon.Results:After modeling,obvious increases in gross score(P＜0.01),histopathological score(P＜0.01),expressions of IL-6 and STAT3(all P＜0.01)of colon were found.After the treatment with sulfasalazine and Gamuzhuer,obvious decreases in gross score(P＜0.01,P＜0.05),histopathological score(all P＜0.01),expressions of IL-6 and STAT3(all P＜0.01)of colon were found.Conclusion:Gamuzhuer enema can reduce the colonic inflammation of colitis model rats and repair the damage of its pathological structure.It may work well in the treatment by regulating down IL-6/STAT3 signal pathway.
GamuzhuerUlcerative colitisInterleukin-6Signal transducer and activator of transcription 3
NETL
NSTL
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