嘎木珠尔灌肠通过对IL-6/STAT3信号通路的调节而改善结肠炎模型大鼠的结肠炎症
Gamuzhuer Ameliorate Colonic Lesions of Colitis Model Rats by Regulating IL-6/STAT3 Signal Pathway
陈浩 1杜阳 1何和平 2侯孝涛 1张丹丹 1赵冉1
作者信息
- 1. 东南大学附属中大医院江北院区,江苏 南京 210044
- 2. 内蒙古自治区国际蒙医医院,内蒙古 呼和浩特 010065
- 折叠
摘要
目的:观察嘎木珠尔灌肠对结肠炎模型大鼠结肠病变的影响;并研究其对IL-6/STAT3信号通路的调节作用.方法:40只大鼠被随机分组;空白组、造模组、柳氮磺吡啶组及嘎木珠尔组,每组10只.以乙醇/TNBS灌肠造模后分别以生理盐水、柳氮磺吡啶及嘎木珠尔灌肠.14天后对结肠大体形态及结肠病理切片进行观察,免疫组化分析结肠IL-6及STAT3的表达.结果:造模后结肠大体评分及组织病理评分均显著增高(P均＜0.01);结肠IL-6及STAT3阳性染色细胞数量均显著增多(P均＜0.01).经柳氮磺吡啶及嘎木珠尔治疗后结肠大体评分均明显降低(P＜0.01、P＜0.05);结肠组织病理评分均显著降低(P均＜0.01);结肠IL-6及STAT3阳性染色细胞数均显著减少(P均＜0.01).结论:嘎木珠尔灌肠能减轻结肠炎模型大鼠的结肠炎症,并可改善其病理结构的破坏.其可能通过对IL-6/STAT3信号通路下调而发挥作用.
Abstract
Objective:To observe the improvement effect of Gamuzhuer enema on colonic lesions of colitis model rats,and to study its effect on IL-6/STAT3 signaling pathway.Methods:40 rats were divided at random into 4 groups:blank,model,sulfasalazine and Gamuzhuer,with 10 in each group.After the model was made by ethanol/TNBS enema;normal saline,sulfasalazine and Gamuzhuer enema were used respectively.After 14 days,the colon was taken for observation with naked eye and microscope.Immunohistochemical analysis was performed to evaluate the expression of IL-6 and STAT3 in colon.Results:After modeling,obvious increases in gross score(P＜0.01),histopathological score(P＜0.01),expressions of IL-6 and STAT3(all P＜0.01)of colon were found.After the treatment with sulfasalazine and Gamuzhuer,obvious decreases in gross score(P＜0.01,P＜0.05),histopathological score(all P＜0.01),expressions of IL-6 and STAT3(all P＜0.01)of colon were found.Conclusion:Gamuzhuer enema can reduce the colonic inflammation of colitis model rats and repair the damage of its pathological structure.It may work well in the treatment by regulating down IL-6/STAT3 signal pathway.
关键词
嘎木珠尔/溃疡性结肠炎/白介素-6/信号转导及转录活化因子3Key words
Gamuzhuer/Ulcerative colitis/Interleukin-6/Signal transducer and activator of transcription 3引用本文复制引用
基金项目
内蒙古自治区卫计委科研课题(201703030)
中国民族医药学会科研项目(2020ZY333-380503)
出版年
2024
NETL
NSTL
万方数据