Objective:To study the optimum purification conditions of total triterpenoids from Rhus chinensis.Methods:Through static and dynamic experiments,the effects of resin type,sample pH value,sample flow rate,sample concentration,sample amount,and elution curve on triterpenoid compounds in Rhus chinensis Mill.were screened.Using the total triterpenoid transfer rate as an indicator,the optimal conditions for impurity removal solvent flow rate,enrichment solvent flow rate,and aspect ratio were optimized using response surface methodology.Results:The optimal purification process conditions are as follows:AB-8 macroporous adsorption resin,with a diameter to height ratio of 1:10,a sample pH of 6.0,a loading volume of 60 mL,a loading concentration of 1.00 mg/mL,and a loading flow rate of 1 BV/h;Concentration,flow rate,and dosage of impurity removal solvent:60％ethanol,2 BV/h,5 BV;Concentration,flow rate,and dosage of enriched solvents:90％ethanol,4 BV/h,5 BV.Validation experiments were conducted under the optimal purification conditions,and the total triterpenoid purification rate was 55.21±0.31％,with a total transfer rate of 98.24±0.87％.Conclusion:AB-8 macroporous adsorption resin can effectively enrich the triterpenoid components in Rhus chinensis Mill.and improve the purity of the active components,providing a foundation for the separation and purification of triterpenoid active components in Rhus chinensis Mill.
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