Study of the Mechanism of Mongolian Medicine Zhenbao Pill in Treating Rheumatoid Arthritis Based on Network Pharmacology and Molecular Docking Techniques
Objective:To explore the possible mechanism of the Mongolian Medicine Zhenbao pill in the treatment of Rheumatoid Arthritis based on the integrated research strategy of network pharmacology and molecular docking.Methods:The active ingredients and targets of Mongolian Medicine Zhenbao Pills were collected and collated through TCMSP,TCM-ID、ETCM、Swiss Target Prediction and related literature databases.The main targets of RA were obtained by Genecards、OMIM、TTD and DisGeNET databases.Meanwhile,the intersection targets of Zhenbao pill and RA were sorted out.Constructing common target protein interaction network by STRING platform.Gene Ontology(GO)term and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were conducted using DAVID database,and Cytoscape3.9.1 software was used to construct the"traditional Chinese medicine-active ingredient-potential target"network,and Autodock software was used to conduct docking between the core compound and target.Results:In this study obtained that 194 active components of Mongolian Medicine Zhenbao pill and 207 intersection targets,and then regulate PI3K-Akt signaling pathway,JAK kinase-signal transducer and activator of transcription pathway and NF-Kb signaling pathway,the aim of RA therapy is to inhibit the angiogenesis of Proinflammatory cytokine and synovium,to promote the production of anti-inflammatory cytokines,to regulate the immune and oxidative stress responses.The results of molecular docking showed that the binding energies of the four key active components to the five core targets were all less than-5 kCal/mol,indicating that the receptors had good binding activity to the ligands.Conclusion:The Mongolian Medicine Zhenbao Pill can treat RA through multi-target and multi-pathway,which can provide a theoretical basis for the further research and clinical application of Zhenbao Pill in the treatment of Rheumatoid Arthritis.
Mongolian Medicine Zhenbao PillRheumatoid ArthritisNetwork PharmacologyMolecular DockingMechanism of Action
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